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Plasmid#135377PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E735 )DepositorInsertnSaCas9 E735 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 G129 miniABEMax V82G
Plasmid#135380PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (G129 )DepositorInsertnSaCas9 G129 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 H119 miniABEMax V82G
Plasmid#135381PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (H119 )DepositorInsertnSaCas9-ABEMax(V82G) H119
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 M732 miniABEMax V82G
Plasmid#135382PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M732 )DepositorInsertnSaCas9 M732 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 M741 miniABEMax V82G
Plasmid#135383PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M741 )DepositorInsertnSaCas9 M741 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 N120 miniABEMax V82G
Plasmid#135384PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N120 )DepositorInsertnSaCas9 N120 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1 1029 miniABEMax V82G strategy1
Plasmid#135360PurposeExpression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminusDepositorInsertmini ABEMax 1029 nSpyCas9
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1 1029 V82G ABEMax Strategy 3
Plasmid#135363PurposeExpression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminusDepositorInsert1029 V82G ABEMax
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTH862-CEN-slowmaxRLuc/minCFLuc
Plasmid#115371PurposeExpresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferaseDepositorInsertsFirefly Luciferase (codon minimised)
Gcn4 5'-UTR + Renilla luciferase (codon optimised)
UseTagsExpressionYeastMutationAll codons have been changed for the most favoura…PromoterADH1 and TDH3Available sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoMaxGFP-CaURA3
Plasmid#44875PurposeYeast tagging vector to create gene fusion with yeast optimized MaxGFP with CaUra3 selectionDepositorInsertyoMaxGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoMaxGFP-SpHis5
Plasmid#44840PurposeYeast tagging vector to create gene fusion with yeast optimized MaxGFP with SpHis5 selectionDepositorInsertyoMaxGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTH748-CEN-RLuc/min8maxCFLuc
Plasmid#38214DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe first eight codons of the original FLuc gene …PromoterADH1 and TDH3 (=GDP)Available sinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH753-CEN-RLuc/min346maxCFLuc
Plasmid#38219DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe first 346 codons of the original FLuc gene we…PromoterADH1 and TDH3 (=GPD)Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH747-CEN-RLuc/min4maxCFLuc
Plasmid#38213DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe first four codons of the original FLuc gene w…PromoterADH1 and TDH3 (=GDP)Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP1-S347A
Plasmid#18993DepositorInsertYes-kinase associated protein S347A mutant (YAP1 Human)
UseTagsHisExpressionMammalianMutationSerine 347 changed to AlaninePromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE2max-∆RNaseH-dualU6
Plasmid#198735PurposeAAV genome encoding C-terminal PE2max and U6 expression cassettesDepositorInsertNpuC-CtermPE2max∆RNaseH
UseAAVTagsExpressionMutationPromoterCbhAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpRYc-P2A-EGFP
Plasmid#208336PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYcDepositorInserthuman codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationSc++ (1-1119), SpRY (1111-1368), D10APromoterCAGAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-PEmax-P2A-Puro
Plasmid#228890PurposeLentiviral delivery and expression of PEmax protein with puromycin selection cassette.DepositorInsertsPEmax
Puromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-PEmax-IRES-ZeoR
Plasmid#207353PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.DepositorInsertPrime editor max (PEmax) enzyme
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX601‐MHP1‐ABEmaxC2‐NG‐E53 ogRNA
Plasmid#187068PurposeGp41-1 Split C-terminal half of ABEmaxNGA driven by MHP1 promoter with gRNA targeting mdx4cv nonsense mutationDepositorInsertGp41-ABEmaxC2‐NG
UseAAVTagsExpressionMammalianMutationPromoterMHP1Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only