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Plasmid#104552Purposebacterial expression of Exonuclease IDepositorInsertExonuclease I (sbcB E.coli)
UseTags6X-His tag followed by thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLM-mCerulean-cMyc
Plasmid#23244DepositorInsertmCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSN001 pME minprom_mCerulean
Plasmid#195959PurposeGateway pME fluorophoreDepositorInsertmouse beta-globin minimal promoter mCerulean
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b-TEVP-cleavableELP
Plasmid#219402PurposeTobacco etch virus protease-responsive elastin-like polypeptide (ELP)DepositorInsertTEVP-cleavableELP
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
hSyn-somBiPOLES-mCerulean
Plasmid#154945PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCK082 desmaMCS:minprommCherry,exorh:mCerulean
Plasmid#200015PurposeExpression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCherry, exorh:mCeruleanDepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
CaMKII-somBiPOLES-mCerulean
Plasmid#154948PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorHas ServiceAAV Retrograde, AAV5, and AAV9InsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterCaMKIIa(0.4)Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mCerulean
Plasmid#99268PurposeContains a fusion of human histone H2B to fluorescent protein mCerulean in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcriptionDepositorInsertmCerulean-3XFLAG
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS-H2B-cerulean
Plasmid#53748PurposeContains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.DepositorInserthistone H2B (H2BC21 Human)
UseTagsCeruleanExpressionMammalianMutationPromotersimian CMV IE94Available sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS-membrane-ceruleanFP
Plasmid#53749PurposeContains membrane localized cerulean fluorescent protein in pCS expression vector.DepositorInsert2x membrane linker
UseTagsCeruleanExpressionMammalianMutationPromotersimian CMV IE94Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl-W-FUGW
Plasmid#166751PurposeExpress mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.DepositorInsertBcl-W (Bcl2l2 Mouse)
UseLentiviralTagsmCerulean3ExpressionMutationPromoterhuman ubiquitin CAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
PEA1-Nuclease-Puro
Plasmid#171992PurposeDelivers all prime editing nuclease components in a single, puromycin selectable plasmidDepositorInsertCbH-Cas9-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
UseTagsExpressionMammalianMutationGC to CA point mutation in SpCas9 to restore nucl…PromoterCMV for Cas9, U6 for gRNAsAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
mDlx-BiPOLES-mCerulean
Plasmid#154946PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVTagsExpressionMammalianMutationPromoterminimal DlxAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cerulean-2xFv-hRIPK3
Plasmid#106079Purposeexpression in mammalian cellsDepositorInsertCerulean-2xFv-hRIPK3 (RIPK3 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK081 desmaMCS:minpromCerulean,exorh:mCherry
Plasmid#200014PurposeExpression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherryDepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
PEA1-Nuclease-GFP
Plasmid#171994PurposeDelivers all prime editing nuclease components in a single, GFP selectable plasmidDepositorInsertCbH-Cas9-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
UseTagsExpressionMammalianMutationGC to CA point mutation in SpCas9 to restore nucl…PromoterCMV for Cas9, U6 for gRNAsAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-PCNA-PuroR
Plasmid#215063PurposeExpresses mCerulean-PCNA in mammalian cells from a retroviral vector.DepositorInsertPCNA (PCNA Human)
UseRetroviralTagsmCeruleanExpressionMammalianMutationPromoterLTRAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCK004 ubi:mCerulean_SV40 (#302)
Plasmid#195978PurposeExpression constructDepositorInsertubi:mCerulean
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
p21-mCerulean-NeoR
Plasmid#215080PurposeExpresses p21-mCerulean in mammalian cells.DepositorInsertCDKN1A (CDKN1A Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-GOS1
Plasmid#18846DepositorInsertGOS1 (GOS1 Budding Yeast)
UseTagsceruleanExpressionYeastMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only