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Showing: 301 - 320 of 573 results

  3. pDR111_mannose_Klf4Gata-3

    Plasmid
    #188401
    Purpose
    Expresses mouse transcription factors Klf4 and Gata-3 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Inserts
    Klf4
    Gata-3
    Use
    Tags
    PhoD secretion peptide
    Expression
    Bacterial
    Mutation
    See Depositor Comments Below
    Promoter
    D-mannose
    Availability
    Academic Institutions and Nonprofits only
    188401-plasmid-map-sequence-id-370467

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  6. pcpAzpaRS_v2

    Plasmid
    #196483
    Purpose
    To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40(synthetase), Pol III tRNA promoter (tRNA), C…
    Availability
    Academic Institutions and Nonprofits only
    196483-plasmid-map-sequence-id-388871

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  7. pcpBpaRS_v2

    Plasmid
    #196485
    Purpose
    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40(synthetase), Pol III tRNA promoter (tRNA), C…
    Availability
    Academic Institutions and Nonprofits only
    196485-plasmid-map-sequence-id-388866

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  8. pVSL10

    Plasmid
    #201182
    Purpose
    Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable
    Depositor
    Jack Szostak
    Article
    Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.
    Insert
    Bst polymerase large fragment (BF) F710Y/D598A
    Use
    Tags
    HRV 3C protease cleavage site and His6
    Expression
    Bacterial
    Mutation
    F710Y D598A
    Promoter
    T5-lacO
    Availability
    Academic Institutions and Nonprofits only
    201182-plasmid-map-sequence-id-406202

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  10. pVR2

    Plasmid
    #84719
    Purpose
    E. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcription
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    12 nt stretch lacking A's inserted before 5&…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    84719-plasmid-map-sequence-id-167481

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  11. pVR2-m4

    Plasmid
    #84723
    Purpose
    pVR2 but with G to U and A to C mutations following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G to U mutation at the first nucleotide after the…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    84723-plasmid-map-sequence-id-167501

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  12. pVR2-m5

    Plasmid
    #84724
    Purpose
    pVR2 but with an insertion of 1 U immediately following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    insertion of 1 U between positions 39 and 40 of a…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    84724-plasmid-map-sequence-id-167505

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  13. pVR2-m6

    Plasmid
    #84725
    Purpose
    pVR2 but with an insertion of 2 U's immediately following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    insertion of 2 Us between positions 39 and 40 of …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    84725-plasmid-map-sequence-id-167510

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  14. pVR2-m7

    Plasmid
    #84726
    Purpose
    pVR2 but with a G to U mutation immediately following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G to U mutation at position 41 of annotated "…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    84726-plasmid-map-sequence-id-167513

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  15. pVR2-m8

    Plasmid
    #84727
    Purpose
    pVR2 but with two G to U mutations immediately following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G to U mutations at positions 40 and 41 of annota…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    84727-plasmid-map-sequence-id-167518

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  16. p1G108CFa

    Plasmid
    #85089
    Purpose
    Expresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 (A74G/C773S/C810S) in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The G108C variant of PCNA1 fused to an FAD domain of P450 BM3
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    G108C in PCNA1, A74G/C773S/C810S in FAD domain of…
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    85089-plasmid-map-sequence-id-164483

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  17. p3R112C/T180CBM3

    Plasmid
    #85090
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The R112C/T180C variant of PCNA3 fused to P450 BM3
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    85090-plasmid-map-sequence-id-164484

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Showing: 301 - 320 of 573 results