pVR2-m2
(Plasmid #84721)

• Purpose: pVR2 but with a G to U mutation in the riboswitch aptamer
• Depositing Lab: Nils Walter
• Publication: Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. doi: 10.1016/j.molcel.2018.08.046.

Backbone

• Vector backbone: pUC18
• Backbone manufacturer: ThermoFisher Scientific
• Backbone size w/o insert (bp): 2686
• Total vector size (bp): 2882
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: E. coli RecA promoter
• Species: E. coli
• Insert Size (bp): 42
• GenBank ID: NC_000913.3 (2,823,819..2,823,860) NC_000913.3

Gene/Insert 2

• Gene/Insert name: stretch lacking A's followed by 5' UTR of queC gene
• Alt name: preQ1 riboswitch
• Species: Bacillus subtilis
• Insert Size (bp): 163
• Mutation: G to U mutation at position 16 of annotated "BSU_misc_RNA_22"
• GenBank ID: NC_000964.3 (1,439,265..1,439,415) NC_000964.3

Resource Information

• Supplemental Documents:
  • annotated sequence
• A portion of this plasmid was derived from a plasmid made by: Catherine Eichhorn at the University of Michigan

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry