Skip to main content
Addgene
Showing: 601 - 640 of 23151 results

  1. lentiCRISPR - EGFP sgRNA 3

    Plasmid
    #51762
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 3
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51762-plasmid-map-sequence-id-79380

    Enlarge

  2. R777-E329 Hs.SPRY2

    Plasmid
    #70613
    Purpose
    Gateway ORF clone of human SPRY2 [NM_005842.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SPRY2 (SPRY2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70613-plasmid-map-sequence-id-126062

    Enlarge

  9. R777-E333 Hs.SPRY4

    Plasmid
    #70618
    Purpose
    Gateway ORF clone of human SPRY4 [NM_001127496.1] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SPRY4 (SPRY4 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70618-plasmid-map-sequence-id-126067

    Enlarge

  11. pLentiCRISPRv2-Stuffer-HepmTurquoise2

    Plasmid
    #192826
    Purpose
    Contains stuffer sequence into which sgRNAs can be cloned and expresses mTurquoise2 from hepatocyte-specific promoter
    Depositor
    Kristin Knouse
    Article
    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do
    Insert
    mTurquoise2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…
    Available since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    192826-plasmid-map-sequence-id-378002

    Enlarge

  13. lentiCRISPR - EGFP sgRNA 6

    Plasmid
    #51765
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 6
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51765-plasmid-map-sequence-id-79389

    Enlarge

  14. R777-E325 Hs.SPRED3

    Plasmid
    #70609
    Purpose
    Gateway ORF clone of human SPRED3 [NM_001042522.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SPRED3 (SPRED3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70609-plasmid-map-sequence-id-126058

    Enlarge

  18. lentiCRISPR - DDX3Y sgRNA 2

    Plasmid
    #70657
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against DDX3Y
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. lentiCRISPR - DDX3Y sgRNA 1

    Plasmid
    #70656
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against DDX3Y
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  32. CRISPR-Sa-TdTom. Reporter

    Plasmid
    #99652
    Purpose
    Red fluorescent reporter that can be activated by dSa Cas9 activator indicating activity
    Depositor
    George Church
    Insert
    RFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    99652-plasmid-map-sequence-id-192053

    Enlarge

  34. R777-E331 Hs.SPRY3

    Plasmid
    #70615
    Purpose
    Gateway ORF clone of human SPRY3 [NM_005840.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SPRY3 (SPRY3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70615-plasmid-map-sequence-id-126064

    Enlarge

  35. 474 p5E-basprom

    Plasmid
    #31158
    Depositor
    Nathan Lawson
    Insert
    basal adenovirus E1b TATA element
    Use
    Entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 11, 2011
    Availability
    Academic Institutions and Nonprofits only
    31158-plasmid-map-sequence-id-37676

    Enlarge

Showing: 601 - 640 of 23151 results