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pHIP_RNA1

Plasmid

#61259

Purpose

Orsay virus RNA1 genome segment expression plasmid for virus reverse genetics

Depositor

David Wang

Article

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA1

Use

Tags

Expression

Worm

Mutation

Promoter

heat shock promoter

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHIP_RNA2FLAG

Plasmid

#61261

Purpose

Flag tagged Orsay virus RNA2 genome segment expression plasmid

Depositor

David Wang

Article

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA2

Use

Tags

Flag fused

Expression

Worm

Mutation

Promoter

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-GFP-OSBP

Plasmid

#134659

Purpose

Flag and GFP-tagged OSBP

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

OSBP (OSBP Human)

Use

Lentiviral

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE PGK-GFP-IRES-LUC-W

Plasmid

#46793

Purpose

2nd generation lentiviral vector

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Inserts

PGK
eGFP (eGFP )
IRES
Luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-C-EGFP

Plasmid

#122844

Purpose

C-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-BCL6-IRES-GFP

Plasmid

#31391

Depositor

Hilda Ye

Article

Yu et al Blood. 2005 Feb 15. 105(4):1777-84.

Insert

B-cell lymphoma 6 (BCL6 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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CMV-eGFP-Z1

Plasmid

#135049

Purpose

Enhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.

Depositor

Jordan Green

Insert

eGFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Blast DEST ERKKTRmRuby2

Plasmid

#90231

Purpose

Lentiviral vector to express ERK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

ERK Kinase Translocation Reporter

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-JAK1

Plasmid

#174572

Purpose

Human FLAG-tagged JAK1 expression (N-term. tag) (CMV prom.)

Depositor

Mathew Garnett

Article

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

JAK1 (JAK1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pDMyc-TNPO1

Plasmid

#86081

Purpose

A mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Transportin-1 (TNPO1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab4a-7

Plasmid

#56440

Purpose

Localization: Ras GTPase, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Rab4a (RAB4A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAAV uN2CpolyGly op GFP (NIID)

Plasmid

#224356

Purpose

Express GFP tagged upsteram ORF of NOTCH2NLC with an expansion (100x) of GGC repeats with codon optimization (NOT pure GGC repeats)

Depositor

Nicolas Charlet-Berguerand

Article

Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

Insert

uN2C with a GGC repeat expansion (100x)

Use

AAV

Tags

eGFP

Expression

Mammalian

Mutation

Codon-optimized for expression in human, so no pu…

Promoter

CMV + chimeric intron (CAG)

Available since

Feb. 5, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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AAVS1-Bxb1-LP-TC

Plasmid

#183754

Purpose

AAVS1 targeting construct with the Bxb1 landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

loxP-eGFP-attP-Bsd-lox257

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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EX-HA-FRB-Cb5_pLX304

Plasmid

#120915

Purpose

Lentiviral vector expressing EX-HA-FRB-Cb5 on mammalian ER membrane

Depositor

Alice Ting

Article

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

EX-HA-FRB-Cb5

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA

Plasmid

#37120

Purpose

DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

TdTomato-EGFP

Use

AAV and Cre/Lox; Cre-switch

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag p38 alpha

Plasmid

#20351

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 alpha (Mapk14 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pHIP_RNA2

Plasmid

#61260

Purpose

Orsay virus RNA2 genome segment expression plasmid for virus reverse genetics

Depositor

David Wang

Article

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA2

Use

Tags

Expression

Worm

Mutation

Promoter

heat shock promoter

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-PGBD5

Plasmid

#78121

Purpose

Lentiviral gene expression vector for doxycycline inducible PGBD5 expression

Depositor

Alex Kentsis

Article

Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

Insert

PGBD5 (PGBD5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pNCS-mClover3

Plasmid

#74236

Purpose

Constitutive expression of mClover3 in bacteria

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Has Service

Cloning Grade DNA

Insert

mClover3

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-mTrim21

Plasmid

#105516

Purpose

Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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