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Showing: 301 - 320 of 144813 results

  1. SP6-hCas9-Ce-mRNA

    Plasmid
    #47911
    Purpose
    In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.
    Depositor
    Paul Sternberg
    Article
    Chiu et al Genetics. 2013 Aug 26.
    Insert
    hCas9 with C. elegans 5' and 3' UTRs
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    SP6
    Availability
    Academic Institutions and Nonprofits only
    47911-plasmid-map-sequence-id-68528

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  2. SP6-sgRNA-scaffold

    Plasmid
    #47912
    Purpose
    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
    Depositor
    Paul Sternberg
    Article
    Chiu et al Genetics. 2013 Aug 26.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    sgRNA 3' end
    Expression
    Worm
    Mutation
    Promoter
    SP6
    Availability
    Academic Institutions and Nonprofits only
    47912-plasmid-map-sequence-id-68534

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  4. pSL1180-HR-PUbECFP

    Plasmid
    #47917
    Purpose
    Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
    Depositor
    Leslie Vosshall
    Insert
    ECFP
    Use
    Tags
    NLS
    Expression
    Insect
    Mutation
    Promoter
    Aedes aegypti polyubiquitin (PUb)
    Availability
    Academic Institutions and Nonprofits only
    47917-plasmid-map-sequence-id-68604

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  14. pIK86

    Plasmid
    #47933
    Purpose
    C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.
    Depositor
    Iskra Katic
    Article
    Katic et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR and Unspecified
    Tags
    Expression
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    47933-plasmid-map-sequence-id-68745

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Showing: 301 - 320 of 144813 results