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Plasmid#170805PurposeAll-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptideDepositorInsertCas9
UseCRISPR and LentiviralTagsNLSExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3b-8XGliBS:EGFP
Plasmid#84602PurposeGli protein-dependent EGFP reporter for analysis of Hedgehog signalingDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoter8X Gli-binding siteAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-Nrf2
Plasmid#67636PurposeAAV vector expressing Nrf2 geneDepositorInsertNrf2 (Nfe2l2 Mouse)
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLL7.0: tgRFPt-SSPB WT
Plasmid#60415PurposeMammalian Expression of tgRFPt-NanoDepositorInserttgRFPt-SSPB
UseCre/Lox and LentiviralTagsTagRFPtExpressionMammalianMutationY11K and A15EPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
5xNF-kb_RE::RedF
Plasmid#124530PurposeNFKB RedFirefly luciferase reporterDepositorInsertNF-κβ RedF reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-DPP4-puro
Plasmid#158451PurposeConstitutive lentiviral expression of human DPP4.DepositorInsertDPP4 (DPP4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cherry-Fibrillarin-C1
Plasmid#134539PurposeExpresses Cherry-Fibrillarin fusion proteinDepositorInsertFibrillarin (FBL Human)
UseTagsCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iGlucoSnFR-TS
Plasmid#127952PurposeExpresses the iGlucoSnFR-TS biosensor for intracellular [glucose], with a fluorescence-lifetime readout, in mammalian cellsDepositorInsertiGlucoSnFR-TS
UseAAVTagsHis6 and Xpress epitopeExpressionMammalianMutationPromoterCAGAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGWB555
Plasmid#74885PurposeGateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.V1
Plasmid#127847Purposenon-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.V1 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF2858
Plasmid#143385PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertID3 (ID3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE-Plus
Plasmid#214079PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dnDepositorInsertPE-Plus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-p53DD-pcDNA3
Plasmid#25989DepositorInserttumor protein p53 (TP53 Human)
UseTagsT7ExpressionMammalianMutationtruncated cDNA encodes truncated protein (aa 300-…PromoterAvailable sinceOct. 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS306-OsTIR1F74A
Plasmid#198416PurposeExpresses OsTIR1F74A under the control of ADH1 promoter in budding yeastDepositorInsertOsTIR1F74A
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFX-β-tubulin-EGFP
Plasmid#175827PurposeExpress EGFP with microtubule-targeting sequence in mammalian cellsDepositorInsertEGFP with microtuble-targeting sequence
UseTagshuman tubulin beta 4B class IVb;TUBB4BExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP(S65T)-His3MX6
Plasmid#41598DepositorInsertHis3
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionYeastMutationPromoterTEFAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFM699
Plasmid#74636Purposealias pFA6a-mCherry-natNT2 - vector for C-terminal tagging of yeast genes with mCherry and nourseothricin selection markerDepositorInsertmCherry
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI-URA3
Plasmid#168055PurposeReplaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymScarletI
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+(rInsp)-Ins-GLuc
Plasmid#89928PurposeExpresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorInserthuman insulin-Gaussia-Luciferase (INS Synthetic, Human)
UseTagsExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…Promoterrat Insulin II promoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-mCherry-GPI
Plasmid#65295Purposesynchronize trafficking of mCherry-GPI from the ER (RUSH system)DepositorInsertGPI anchor
UseTagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only