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p-Tet-O-EYA2-Puro

Plasmid

#221669

Purpose

Expresses EYA2 in mammalian cells

Depositor

Vanessa Hall

Article

Bergmann et al Front Cell Dev Biol. 2022 Aug 15;10:9765

Insert

EYA2 (EYA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-ChloC-P2A-eGFP

Plasmid

#179840

Purpose

donor plasmid for cardiac-specific CCND2-T2A-Chloc-P2A-eGFP expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

chloride-conducting ChR (ChloC) and eGFP

Expression

Mammalian

Mutation

Promoter

Troponin T promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-CASK

Plasmid

#221427

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

CASK (CASK Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ARID1B(23))-PGKpuro2ABFP-W

Plasmid

#200470

Purpose

Lentiviral vector expressing gRNA targeting human ARID1B

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ARID1B(23) (ARID1B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ARID1B(21))-PGKpuro2ABFP-W

Plasmid

#200469

Purpose

Lentiviral vector expressing gRNA targeting human ARID1B

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ARID1B(21) (ARID1B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO shLuc/FLAG Metap2

Plasmid

#209316

Purpose

Lentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA)

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

METAP2 (METAP2 Human, human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ARID1B_QKI

Plasmid

#205771

Purpose

Express mEGFP-tagged fusion protein, ARID1B_QKI from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ARID1B_QKI (ARID1B, QKI Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-DNAH7 [N250/21R]

Plasmid

#206547

Purpose

Mammalian Expression Plasmid of anti-DNAH7 (Human). Derived from hybridoma N250/21.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-DNAH7 (Homo sapiens) recombinant Mouse monoclonal antibody (DNAH7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Kv1.7 K+ channel scFv [N471/27]

Plasmid

#206753

Purpose

Mammalian Expression of Kv1.7 K+ channel scFV. Derived from hybridoma N471/27 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv1.7 K+ channel (Mus musculus) recombinant scFV (Kcna7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Kv4.2 K+ channel (external) scFv [K57/41]

Plasmid

#206771

Purpose

Mammalian Expression of Kv4.2 K+ channel (external) scFV. Derived from hybridoma K57/41 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv4.2 K+ channel (external) (Homo sapiens) recombinant scFV (KCND2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(deltaI)

Plasmid

#204701

Purpose

This plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-p300(deltaI) (BRD4, EP300 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

deletion of intrinsically disordered regions of p…

Promoter

CMV

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Kv1.7 K+ channel [N471/27R]

Plasmid

#199400

Purpose

Mammalian expression plasmid of Anti-Kv1.7 K+ channel [N471/27R] (Rat). Derived from hybridoma N471/27.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Kv1.7 K+ channel (Rat) recombinant mouse monoclonal antibody (Kcna7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(MIN)

Plasmid

#204695

Purpose

This plasmid allows for inducible expression of BRD4-NUT(MIN) mutant fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-NUT(MIN) (BRD4, NUTM1 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

deletion of majority of NUT, retaining only amino…

Promoter

CMV

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(I)

Plasmid

#204700

Purpose

This plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-p300(I) (BRD4, EP300 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

deletion of all TF-bidning domains and HAT in p300

Promoter

CMV

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(HI)

Plasmid

#204699

Purpose

This plasmid allows for inducible expression of engineered BRD4-p300(HI) fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-p300 (BRD4, EP300 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

deletion of all TF-bidning domains in p300

Promoter

CMV

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(H*IT)

Plasmid

#204698

Purpose

This plasmid allows for inducible expression of engineered BRD4-p300(H*IT) fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-p300(H*IT) (BRD4, EP300 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

D1399Y substitution in p300

Promoter

CMV

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-JAG2-COMP5AP-AviTag-9xHis

Plasmid

#157591

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

JAG2 (JAG2 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-IGSF8-COMP5AP-AviTag-9xHis

Plasmid

#157476

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IGSF8 (IGSF8 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-FGFR2-COMP5AP-AviTag-9xHis

Plasmid

#157365

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

FGFR2 (FGFR2 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-HAPLN4-COMP5AP-AviTag-9xHis

Plasmid

#157373

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

HAPLN4 (HAPLN4 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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