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Plasmid#41816PurposeExpresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineeringDepositorInsertCas9_D10A
UseCRISPRTagsExpressionMammalianMutationhuman codon-optimized, D10A nickasePromoterCMVAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC
Plasmid#183790PurposeA single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.DepositorInsertsP2A-eGFP-TargetBC-5xTAPE-1
pegRNA-InsertBC
UseSynthetic BiologyTagsExpressionMutationG>A in the gRNA scaffold- please see depositor…PromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCyRFP1-C1
Plasmid#84355PurposeExpresses mCyRFP1 fluorescent protein in an empty backboneDepositorInsertmCyRFP1
UseTagsExpressionMammalianMutationA161K and N41A, reletive to CyRFP1PromoterCMVAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-rtTA-IRES-eGFP-pGK-NeoR
Plasmid#204497PurposeExpresses rtTA and Neomycin resistance genes in mammalian cellsDepositorInsertrtTA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgRosa26
Plasmid#159914PurposeMutagenesis of Rosa26 with SauCas9DepositorInsertRosa26 gRNA (Gt(ROSA)26Sor Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1a-MICB*005-IRES-ZsGreen
Plasmid#114008PurposeInduces expression of human MICB allele 005 cDNADepositorInsertMICB*005 (MICB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-eGFP
Plasmid#99375PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-eGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
psd44-iGFP-MFGE8-long
Plasmid#46847Purposeexpression of MFGE8 in mammalian cellsDepositorInsertMFGE8 (Mfge8 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGK promoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miniGFP1
Plasmid#188896PurposeExpress 6xHis-Tag miniGFP1 in E.coliDepositorInsertminiGFP1
UseTags6xHisExpressionBacterialMutationPromoteraraBADAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
xCas9(3.7)-ABE(7.10)
Plasmid#108382PurposeMammalian expression vector for xCas9(3.7)-ABEDepositorInsertxCas9(3.7)-ABE(7.10)
UseTagsNLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHPyV7-713a
Plasmid#24728DepositorInsertFull genome of Human polyomavirus 7
UseViral cloneTagsExpressionMutationPromoterAvailable sinceJune 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUCDMp10
Plasmid#170461PurposeEarlyBac insect cell expression systemDepositorInsertNew vector
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMV306hsp+FFluc
Plasmid#26156DepositorInsertFirefly luciferase
UseTagsExpressionBacterialMutationFirefly luciferase gene codon optimised for mycob…PromoterAvailable sinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMON-HsNMT1
Plasmid#67475PurposeExpression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteriaDepositorInsertHsNMT1 (NMT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
3xflag-APEX2-LRRK2
Plasmid#164622PurposeExpresses LRRK2 tagged with APEX2 and 3xFLAGDepositorInsertLRRK2 (LRRK2 Human)
UseTags3xFLAG and APEX2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAT32
Plasmid#204297PurposepJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAV1K-T5-gfp
Plasmid#26702DepositorInsertT5lacOlacOeGFP biobrick
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Hygro DEST ERKKTRmCerulean3
Plasmid#90229PurposeLentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsmCerulean3ExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPN265NB-LaG16-MNase
Plasmid#170978PurposeExpresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & RunDepositorInsertGlutathione-S-Transferase/LaG16/MNase
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B4
Plasmid#127849Purposenon-standard AAV2 rep-AAV-PHP.B4 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B4 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET24b-APEX2
Plasmid#111702PurposeFor IPTG-inducible expression of APEX2 in E. coliDepositorInsertAPEX2
UseTags6xHis and V5ExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)
Plasmid#128605PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetO-hNGN2-eGFP-Puro
Plasmid#79823PurposeExpresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.DepositorInserthNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
UseLentiviralTagsExpressionMutationPromoterTetOAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAcFRS.1.t1
Plasmid#73545PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAcFRS.1.t1
pAcFRS.1.t1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-sh-hSC
Plasmid#46896Purposeexpression of scrambled shRNADepositorInsertscrambled
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-AF8
Plasmid#117803Purposemammalian expression of GFP-tagged human centromere protein ADepositorInsertCENP-A (CENPA Human)
UseTagsGFPExpressionMammalianMutationSee depositor comments below.PromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
p156RRL-EF1a-GFP-U3H1SatA
Plasmid#41795DepositorInsertsatellite repeat sequence
UseTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCR8 JARID2
Plasmid#114443PurposeEntry vector for gateway cloning containing human JARID2 coding sequenceDepositorInsertJARID2 (JARID2 Human)
UseGateway entry cloningTagsExpressionMutationPromoterNoneAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF2409
Plasmid#144021PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXF2 (FOXF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i3 G183S ONE-GO
Plasmid#204338PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-i3 G183S ONE-GO
UseLentiviralTagsExpressionMutationG183S mutation in Galpha-i3 (renders it RGS insen…PromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA
Plasmid#20299PurposeCre-activated AAV expression of mCherryDepositorInsertmCherry
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
PGK-DTAG-CTERM-GFP
Plasmid#185770PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGExpressionMutationnonePromoterPGKAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
p CIneo-RL
Plasmid#115366PurposeExpression vector to produce humanized Renilla luciferaseDepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA
Plasmid#171385PurposeExpression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.DepositorInserthumanized minidCas13X.1-RESCUE-S
UseTagsExpressionMammalianMutationPromoterCMV, U6Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH1-CMV-FIP200-mCherry-Hygro
Plasmid#210852PurposeLentiviral expression vector encoding FIP200-mCherryDepositorInsertFIP200 (RB1CC1 Human)
UseLentiviralTagsmCherryExpressionMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-GFP (PX458)
Plasmid#129026Purposeinactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNADepositorInsertdCas9-hudTET1CD, SgRNA cloning site
UseTagsHA-Tag, NLS and T2A-EGFPExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomCherry-Kan
Plasmid#44903PurposeYeast tagging vector to create gene fusion with yeast optimized mCherry with Kan selectionDepositorInsertyomCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.NES.jRCaMP1a.WPRE.SV40
Plasmid#100848PurposeAAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV1 and AAV9InsertjRCaMP1a
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB1 (CK2alpha)
Plasmid#27083DepositorInsertCK2alpha (CSNK2A1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMOS028: Mitycam calcium sensor (mitochondrial)
Plasmid#163046PurposeMitycam calcium sensor (mitochondrial)DepositorInsertMitycam
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
221-AID-RPB1
Plasmid#195108PurposeTargeting vector to introduce AID tag in N-terminal of the human RPB1 gene. Auxin-inducible degron system.DepositorInsertAID
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
EB3N-VVDfast-mCl3-iLID
Plasmid#190165PurposeExpresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.DepositorInsertEB3N-VVDfast-mClover3-iLID
UseTags-VVDfast-mClover3-iLIDExpressionMammalianMutation1-189 aa from EB3PromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
HC25 pComb3xss anti-Homer1 nanobody
Plasmid#134718PurposeBacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-Homer1 (Mouse) recombinant llama nanobody HC25 (Homer1 L. glama (llama))
UseTags6xHis-HAExpressionBacterialMutationPromoterlacAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIS.MTRIA-PGE
Plasmid#184617PurposeFor expression of a fluorescent sensor for prostaglandin E2 factor in mammalian cellsDepositorInsertMTRIA-PGE
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-RhoA-T19N
Plasmid#12967DepositorInsertRhoA DN (RHOA Human)
UseTagsEGFPExpressionMammalianMutationT19N Dominant negativePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET3a-hUBA1
Plasmid#63571PurposeExpression of human UBA1 tagged with HisDepositorInsertUBA1 (UBA1 Human)
UseTagsExpressionBacterialMutationIsoform 2 (1018 amino acids)PromoterT7Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA-BSD
Plasmid#175583PurposeExpresses sgRNA for CasRx in mammalian cellDepositorInsertsgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
LPAR5-Tango
Plasmid#66421PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertLPAR5 (LPAR5 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pESF140_HisSpy_DDB1∆B
Plasmid#124213PurposeInsect cell expression vector for His6, Spy tagged hsDDB1∆BDepositorInsertDNA damage-binding protein 1 (DDB1 Human)
UseTagsHis6-SpyExpressionInsectMutationinternal deletion of BPB domain to aid crystalliz…PromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -