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mCherry-LaminB1-10

Plasmid

#55069

Purpose

Localization: Nuclear Envelope, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

LaminB1 (LMNB1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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eGFP STAT1 S727A

Plasmid

#12304

Depositor

Alan Perantoni

Article

Timofeeva et al Oncogene. 2006 Jun 26. ():.

Insert

STAT1 S727A (STAT1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

S727A

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pBS-Int

Plasmid

#50000

Purpose

Expresses the integrase on a suicide plasmid

Depositor

Brian Robertson, Peter Sander

Article

Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

Insert

Integrase from pMV361

Use

Tags

Expression

Bacterial

Mutation

Promoter

own

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pGEX Syt1 C2AB (96-421)

Plasmid

#170643

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_miRFP670

Plasmid

#113726

Purpose

Expresses the fluorescent protein miRFP670 in a lentiviral backbone

Depositor

Vanessa LaPointe

Insert

miRFP670

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-C1-CAMKIIalpha

Plasmid

#21226

Depositor

Tobias Meyer

Article

Shen et al Neuron. 1998 Sep . 21(3):593-606.

Insert

CAMKIIalpha (Camk2a Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Lab plasmid KS 0001.

Promoter

Available since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt1 WT IRES mRuby3

Plasmid

#170639

Purpose

Expression of syt1 in cultured neurons by lentiviral transduction

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt1 D303G IRES mRuby3

Plasmid

#170640

Purpose

Expression of syt1 in cultured neurons by lentiviral transduction

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Use

Lentiviral

Tags

Expression

Mutation

D303G

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt1 D365E IRES mRuby3

Plasmid

#170641

Purpose

Expression of syt1 in cultured neurons by lentiviral transduction

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Use

Lentiviral

Tags

Expression

Mutation

D365E

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) D303G

Plasmid

#170646

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Use

Tags

Expression

Bacterial

Mutation

D303G

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) D365E

Plasmid

#170649

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Use

Tags

Expression

Bacterial

Mutation

D365E

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) I367T

Plasmid

#170652

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Use

Tags

Expression

Bacterial

Mutation

I367T

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHD-T2A-split-intein-Gal4[1-20]-N-int

Plasmid

#199203

Purpose

To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHD-T2A-split-intein-Gal4[21-881]-C-int

Plasmid

#199204

Purpose

To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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NG-ABE8e

Plasmid

#138491

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9 (NG)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8m

Plasmid

#192617

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEMG-GRIS

Plasmid

#225134

Purpose

For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.

Depositor

Lars Blank

Article

Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRGEB31

Plasmid

#51295

Purpose

Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plant. by agrobacterium mediated transformation.

Depositor

Yinong Yang

Article

Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1

Type

Empty backbone

Use

CRISPR; Plant expression

Tags

Expression

Plant

Mutation

Promoter

Rice snoRNA U3 and dual 35S promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP6

Plasmid

#18065

Depositor

Edward Yeh

Article

Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

Insert

SENP6 (SENP6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
Non tagged Avidin

Plasmid

#214835

Purpose

This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al bioRxiv 2024.01.21.576499.

Insert

Non tagged Avidin

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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