Addgene

Showing: 3181 - 3200 of 156588 results

Results per page:

Sort results by:

VE-cad KI gRNA1

Plasmid

#92310

Purpose

CRISPR-GFP-gRNA for cutting VEcad

Depositor

Sean Palecek

Article

Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

Insert

VEcad KI gRNA1 (CDH5 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PT7lacO-EF-Ts-His6

Plasmid

#224371

Purpose

Express EF-Ts protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

EF-Ts (tsf E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PT7lacO-EF-G-His8

Plasmid

#224369

Purpose

Express EF-G protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

EF-G (fusA E. coli)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PT7lacO-His6-IF2

Plasmid

#224367

Purpose

Express IF2 protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

IF2 (infB E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PT7lacO-RF3-His6

Plasmid

#224373

Purpose

Express RF3 protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

RF3 (prfB E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PT7lacO-EF-Tu-His8

Plasmid

#224370

Purpose

Express EF-Tu protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

EF-Tu (tufB E. coli)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PT7lacO-His6-PheRSa-PheRSb

Plasmid

#224364

Purpose

Express PheRS protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

PheRS (pheS E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PT7lacO-His6-AsnRS

Plasmid

#224363

Purpose

Express AsnRS protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

AsnRS (asnS E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PT7lacO-RRF-His6

Plasmid

#224374

Purpose

Express RRF protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

RRF (frr E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PT7lacO-His6-IF3

Plasmid

#224368

Purpose

Express IF3 protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

IF3 (infC E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PT7lacO-His6-IF1

Plasmid

#224366

Purpose

Express IF1 protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

IF1 (infA E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PT7lacO-His6-ThrRS

Plasmid

#224365

Purpose

Express ThrRS protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

ThrRS (thrS E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PT7lacO-His6-CKM

Plasmid

#224375

Purpose

Express CKM protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

CKM (LOC107051134 Chicken)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PT7lacO-His6-AlaRS

Plasmid

#224362

Purpose

Express AlaRS protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

AlaRS (alaS E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PT7lacO-His6-NDK

Plasmid

#224376

Purpose

Express NDK protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

NDK (ndk E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro

Plasmid

#211468

Purpose

Lentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

4xGFP11-SEC61B

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-EN1201

Plasmid

#92144

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PLKO.1-G3BP1-CDS

Plasmid

#136039

Purpose

G3BP1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CGGGAATTTGTGAGACAGTAT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

Plasmid

#171385

Purpose

Expression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized minidCas13X.1-RESCUE-S

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV, U6

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEx-NTOM20-mVenus-Zdk1

Plasmid

#81010

Purpose

Expression of Zdk1 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Use

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge