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pMBP-HIS2-Snf7

Plasmid

#21492

Depositor

James Hurley

Article

Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

Insert

SNF7 (SNF7 Budding Yeast)

Use

Tags

6x His and MBP

Expression

Bacterial

Mutation

none

Promoter

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-nEF-Con/Fon-NpHR3.3-EYFP

Plasmid

#137152

Purpose

Intersectional viral expression of NpHR3.3-EYFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-NpHR3.3-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

W179F

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast

Plasmid

#205565

Purpose

Express human NEDD4L (delta C2 isoform)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

NEDD4L (NEDD4L Human)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

delta C2 isoform

Promoter

EF1

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLX208_CMV::mCherry-myc-VAMP2

Plasmid

#199716

Purpose

Expresses VAMP2, fused to mCherry, in mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

Depositor

Alice Ting

Article

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

mCherry-myc-VAMP2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGCP123-EbpA_g1

Plasmid

#153517

Purpose

sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

pnisA-sgRNA(EbpA_g1)-dCas9 scaffold

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTEC22

Plasmid

#30181

Depositor

Lalita Ramakrishnan

Article

Takaki et al Cell Rep. 2012 Jul 26;2(1):175-84. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

tdKatuschka2

Expression

Mutation

Promoter

Available since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

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Chx10BP-LCL-EGFP

Plasmid

#73992

Purpose

Cre-dependent reporter plasmid for bipolar cells in the retina

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

Chx10BP-loxP-mCherry-loxP-EGFP

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Chx10BP (chx10 enhancer)

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-p15

Plasmid

#62656

Purpose

Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

Depositor

Kristala Prather

Article

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

exo, beta, gam, sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3 (human)

Plasmid

#24920

Purpose

Mammalian expression of LC3 fused to EGFP

Depositor

Toren Finkel

Article

Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

Insert

LC3 (MAP1LC3B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pMXs-p53DD

Plasmid

#22729

Depositor

Shinya Yamanaka

Article

Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

Insert

Trp53 (Trp53 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

deleted amino acids 14-301

Promoter

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA

Plasmid

#205317

Purpose

All-in-one AAV vector expressing ISYmu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

humanized ISYmu1 TnpB

Use

AAV

Tags

3 x HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLV puro Tet VSVG

Plasmid

#82724

Purpose

Lentiviral plasmid that expresses VSVG protein under a doxycycline-inducible promoter

Depositor

Warren Zipfel

Insert

VSVG

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV_CMV::hTfR-GFP

Plasmid

#199714

Purpose

Expresses a chimera of human transferrin receptor (hTfR) and GFP in mammalian cells.

Depositor

Alice Ting

Article

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

hTfR-GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pPtPBR11

Plasmid

#80386

Purpose

Episome vector for Phaeodactylum tricornutum (pBR322-based)

Depositor

Philip Weyman

Type

Empty backbone

Use

Synthetic Biology; : episomal vector for phaeodac…

Tags

Expression

Mutation

Promoter

Available since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl2-Cb5

Plasmid

#18000

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

ER targeted Bcl-2 (BCL2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Bcl-2's C terminal targeting sequence replac…

Promoter

CMV

Available since

June 4, 2008

Availability

Academic Institutions and Nonprofits only
pCAGGS_L_EBOV

Plasmid

#103052

Purpose

Encodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

L gene of Ebola virus (L Zaire ebolavirus, Mayinga)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEVOL-pBpF

Plasmid

#31190

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.

Depositor

Peter Schultz

Article

Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99

Insert

M.j. p-benzoylphenylalanine RS (2 copies +tRNA)

Use

Tags

Expression

Bacterial

Mutation

Y32G, E107P, D158T, I159S

Promoter

Available since

Dec. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-BSD

Plasmid

#174821

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-BSD

Use

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl2-Maob

Plasmid

#18001

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Mitochondria-targeted Bcl-2 (BCL2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Bcl-2's C terminal replaced with mitochondri…

Promoter

CMV

Available since

June 4, 2008

Availability

Academic Institutions and Nonprofits only
Perox-GA

Plasmid

#209868

Purpose

Dimerization dependent fluorescent protein GA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP A

Use

Tags

Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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