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Plasmid#190550PurposeMammalian Expression of Beta4-spectrin scFV. Derived from hybridoma N393/2.DepositorInsertBeta4-spectrin (Homo sapiens) recombinant scFV (SPTBN4 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mouse c-MycV394D-IRES-RFP
Plasmid#73573PurposeMammalian retroviral expression of mouse Myc V394D mutantDepositorInsertcMyc (Myc Mouse)
UseRetroviralTagsIRES-RFPExpressionMammalianMutationV394DPromoterMSCVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-SNAPf-BICDR1
Plasmid#111858PurposeN-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDR1 (Bicdl1 Mouse)
UseTagsHis-tag, SNAPf-tag, TEV site, and ZZExpressionInsectMutationR250Q, Optimised for Sf9 expressionPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSIRIP-puro shRNA p19-1
Plasmid#14089DepositorInsertp19 ARF shRNA - 1 (Cdkn2a Mouse)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGIPz-mPD-L1 3SA
Plasmid#121489PurposeExpression of mouse PD-L1 3SADepositorInsertPD-L1 3SA
UseLentiviralTagsFlagExpressionMammalianMutationS175A, T179A, S183APromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xEGFP-mMeikin
Plasmid#174708PurposeMammalian expression of mouse MeikinDepositorInsertMeikin (Meikin Mouse)
UseTags3xEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-mouse-full-length-CHMP3-HA
Plasmid#154176Purposeexpresses mouse CHMP3 in mammalian cellsDepositorInsertCHMP3 (Chmp3 Mouse)
UseTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX335-EN475
Plasmid#86231PurposeFor transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.DepositorInsertspCas9-nickase and sgRNA against mouse CTCF STOP Codon
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-Caspase-1_enzymatic-domain
Plasmid#183385PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-1 (AA130-404) (Casp1 Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7a1 (glu)
Plasmid#14540DepositorInsertMKK7a1(Glu) (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationSer-198Glu Thr-202GluPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-sypHTomato
Plasmid#164613PurposeExpress the pHTomato inserted in the synaptophysion in mammalian cellsDepositorInsertA reporter of vesicular turnover sypHTomato
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-CMV10-Flag-mMcl-1
Plasmid#32978DepositorInsertMcl-1 (Mcl1 Mouse)
UseTags3XFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-Otx2shRNA1
Plasmid#73979PurposeOtx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse Otx2 gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Neo-GFP/Foxa1
Plasmid#105506PurposeMSCV-driven retroviral Foxa1 expressionDepositorInsertFoxa1 (Foxa1 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV4Neo-murine IL-17RA-HA
Plasmid#46859Purposeexpresses mouse IL-17RADepositorInsertIL-17RA (Il17ra Mouse)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-p53DD
Plasmid#196582PurposeMammalian expression of SpCas9 prime editor 2 with P2A-p53DDDepositorInsertPE2-P2A-mp53DD
UseTagsExpressionMammalianMutationΔ40-903PromoterCMVAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCALPs-HA-NFAT1 (4-460)-GFP
Plasmid#88879PurposeLentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFPDepositorInsertHA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
UseLentiviralTagsGFP fusion protein and HA tagExpressionMammalianMutationamino acids 4-460 only; L78P & L287P *see bel…PromoterSFFV (spleen focus forming virus)Available sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-CACNA1E KI
Plasmid#131481PurposeEndogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
p060 pCS mTRPS1
Plasmid#11032DepositorInsertTRPS1 (Trps1 Mouse)
UseXenopus, mammalian, avian, and zebrafishTagsExpressionMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -