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pCMS-dsRed2-C1824U LMNA-GFP minigene

Plasmid

#128231

Purpose

C1824U mutation Lamin A splicing reporter

Depositor

Tom Misteli

Article

Shilo et al Nucleic Acids Res. 2019 Apr 22. pii: 547

Insert

lamin A (LMNA Human)

Use

Tags

GFP and SV40-driven dsRed2 to identify transfecte…

Expression

Mammalian

Mutation

C1824U

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRD4_WT

Plasmid

#82323

Purpose

Gateway Donor vector containing BRD4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRD4 (BRD4 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

P474L

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-p300

Plasmid

#204697

Purpose

This plasmid allows for inducible expression of engineered BRD4-p300 fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-p300 (BRD4, EP300 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

direct fusion of BRD4 to p300

Promoter

CMV

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ARID1B_ZNF384

Plasmid

#205772

Purpose

Express mEGFP-tagged fusion protein, ARID1B_ZNF384 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ARID1B_ZNF384 (ARID1B, ZNF384 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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JDW 836 (pCS2-V5-mScarlet-I-hsKRAS4A-WT)

Plasmid

#156411

Purpose

pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4A-WT (KRAS Human)

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Epsilon [N413/67R]

Plasmid

#114478

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Epsilon (Human). Derived from hybridoma N413/67.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GABA(A)R, Epsilon (Homo sapiens) recombinant mouse monoclonal antibody (GABRE Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Kv4.2 K+ Channel (External) [K57/1R]

Plasmid

#128622

Purpose

Mammalian Expression Plasmid of anti-Kv4.2 K+ Channel (External) (Human). Derived from hybridoma K57/1.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv4.2 K+ Channel (External) (Human) recombinant mouse monoclonal antibody (KCND2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-MND-HsMAN1C1-1-630-cMycFlag-hPGK-GFP

Plasmid

#183505

Purpose

Express GFP and MAN1C1 proteins

Depositor

Olivier Guipaud

Article

Ladaigue et al iScience. 2022 Nov 2;25(12):105482. doi:

Inserts

man1C1 (MAN1C1 Human)
EGFP

Use

Lentiviral

Tags

Myc-DDK

Expression

Mammalian

Mutation

Promoter

MND and hPGK

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) FLAG-Ago2 S387E

Plasmid

#60653

Purpose

Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

CMV-FLAG-Ago2 (N-term tag)

Expression

Mammalian

Mutation

S387E mutant

Promoter

CMV

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.puro_shGLS

Plasmid

#110335

Purpose

shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene, puromycin selection

Depositor

Sandra Martha Gomes Dias

Article

Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

Insert

GLS glutaminase (GLS Human, Synthetic)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6 (RNA PolIII)

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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JDW 763 (pCS2-EYFP-hsKRAS4B-WT)

Plasmid

#156408

Purpose

pCS2 CMV expression vector driving EYFP fused human KRAS4B

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4B-WT (KRAS Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_ACTG1_WT

Plasmid

#81912

Purpose

Gateway Donor vector containing ACTG1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ACTG1 (ACTG1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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L-CRISPR-CTN (MLL-ENL)

Plasmid

#69212

Purpose

Lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-mNeonGreen

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) FLAG-Ago2 S387A

Plasmid

#60652

Purpose

Expresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

CMV-FLAG-Ago2 (N-term tag)

Expression

Mutation

S387A mutant

Promoter

CMV

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-sgArid1b#2/Cre

Plasmid

#173574

Purpose

Expresses a Arid1b-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Arid1b (Arid1b Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgArid1b#1/Cre

Plasmid

#173573

Purpose

Expresses a Arid1b-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Arid1b (Arid1b Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE-2xFLAG-2xSTREP-CCND2-Puro

Plasmid

#172628

Purpose

Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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anti-Jagged_D5-pBIOCAM5

Plasmid

#39345

Depositor

John McCafferty

Article

Schofield et al Genome Biol. 2007;8(11):R254.

Insert

scFv specific for Jagged (clone D5) (JAG1 Human)

Use

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CEACAM1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156826

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CEACAM1 (CEACAM1 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-TRPML3/Mucolipin-3 [N268/19R]

Plasmid

#114477

Purpose

Mammalian Expression Plasmid of anti-TRPML3/Mucolipin-3 (Human). Derived from hybridoma N268/19.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TRPML3/Mucolipin-3 (Homo sapiens) recombinant mouse monoclonal antibody (MCOLN3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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