pTRIP-MND-HsMAN1C1-1-630-cMycFlag-hPGK-GFP
(Plasmid #183505)

• Purpose: Express GFP and MAN1C1 proteins
• Depositing Lab: Olivier Guipaud
• Publication: Ladaigue et al iScience. 2022 Nov 2;25(12):105482. doi: 10.1016/j.isci.2022.105482. eCollection 2022 Dec 22.

Backbone

• Vector backbone: pTRIP
• Total vector size (bp): 12890
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: man1C1
• Alt name: class I alpha-mannosidase C1
• Species: H. sapiens (human)
• Insert Size (bp): 1890
• GenBank ID: NM_020379 NM_020379.3
• Entrez Gene: MAN1C1 (a.k.a. HMIC, MAN1A3, MAN1C, pp6318)
• Promoter: MND
• Tag / Fusion Protein:
  • Myc-DDK (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: 5’CTCACTCGGCGCGATCTGGATCTGCCGCCGCGATCG3’ (Forward)
• 3′ sequencing primer: 5’CCCAACCCCGTGGGAATTCGTTAAACCTTATCGTCGTCATCCTTG3’ (Reverse)

Gene/Insert 2

• Gene/Insert name: EGFP
• Insert Size (bp): 239
• Promoter: hPGK

Cloning Information for Gene/Insert 2

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: 5’CACTCGGCGCGATCTGGATCCCGAATTCCCACGGGGTTG3’ (Forward)
• 3′ sequencing primer: 5’CACCATGGTGGCGACCGGTGGCTGGGGAGAGAGGTCGG3’ (Reverse)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: man1c1: from pCMV6-HsMAN1C1-1-630-cMycFlag plasmid (Origene, Cat#: RC222009)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry