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pGL4-eIF3B MBE3 mutant

Plasmid

#133310

Purpose

Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3

Depositor

Josep Domingo-Domenech

Article

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter MBE3 mutant (EIF3B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

ccacgtgacc changed to cAaAAAAacc

Promoter

eIF3B

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE1 (minus strand)

Plasmid

#91842

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0619)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE1 (minus strand) (CD69 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Perfect match to reference sequence

Promoter

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE2 (minus strand)

Plasmid

#91843

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0620)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE2 (minus strand) (CD69 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Perfect match to reference sequence

Promoter

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE2 (plus strand)

Plasmid

#91848

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0625)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE2 (plus strand) (CD69 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Perfect match to reference sequence

Promoter

Availability

Academic Institutions and Nonprofits only

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BTV

Plasmid

#131589

Purpose

Plasmid for the targeting of BAC clone

Depositor

Joon Tae Park

Insert

Firefly Luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pGL3-FLIP pr4

Plasmid

#19129

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

FLIP promoter (CFLAR Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The sequence corresponds to positions -503 to +10…

Promoter

Availability

Academic Institutions and Nonprofits only
pGL3-FLIP pr5

Plasmid

#19130

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

FLIP promoter (CFLAR Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The sequence corresponds to positions -503 to +28…

Promoter

Availability

Academic Institutions and Nonprofits only

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pGL-p21UTRm1&2

Plasmid

#20880

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p21 3'UTR site 1 and 2 mutation (Cdkn1a Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Predicted miRNA binding site 1 (Position ~420) GC…

Promoter

Availability

Academic Institutions and Nonprofits only
pGL3b(1454/3172)P2P-wt

Plasmid

#21404

Depositor

Eric Metzen

Article

Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

Insert

EGLN1 (EGLN1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pGL3b(1454/3172)P2P-mut

Plasmid

#21405

Depositor

Eric Metzen

Article

Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

Insert

EGLN1 (EGLN1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

hypoxia response element mutated

Promoter

Availability

Academic Institutions and Nonprofits only
pGL3-p27 T187A Luc

Plasmid

#23048

Depositor

William Kaelin

Article

Zhang et al Nat Med. 2004 Jun . 10(6):643-8.

Insert

p27 (CDKN1B Human)

Use

Tags

Luciferase

Expression

Mammalian

Mutation

T187A: mutation at codon 187 changing from T (ACG…

Promoter

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-YNL-CP

Plasmid

#65714

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-CNL-CP

Plasmid

#65715

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-ONL-CP

Plasmid

#65716

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pFrt-invCAG-Luc

Plasmid

#63577

Purpose

Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sites

Depositor

Ivo Huijbers

Article

Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

Insert

Stop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG

Use

Cre/Lox; Frt/flpe recombination mediated insertion

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Luc-Nanog-3'UTR

Plasmid

#63893

Purpose

Luciferase reporter of mouse wildtype Nanog 3'UTR

Depositor

Lin He

Article

Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

Insert

Nanog homeobox (Nanog Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Luc-Nanog-3'UTRmut

Plasmid

#63894

Purpose

Luciferase reporter of mouse Nanog 3'UTR with the miR-34 target site mutated

Depositor

Lin He

Article

Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

Insert

Nanog homeobox (Nanog Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
Luc-Sox2-3'UTR

Plasmid

#63895

Purpose

Luciferase reporter of mouse wildtype Sox2 3'UTR

Depositor

Lin He

Article

Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

Insert

SRY (sex determining region Y)-box 2 (Sox2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pGL3-Cp110-3'UTR

Plasmid

#64083

Purpose

Luciferase assay

Depositor

Lin He

Article

Song et al Nature. 2014 Jun 5;510(7503):115-20. doi

Insert

Ccp110 (centriolar coiled coil protein 110) (Ccp110 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pmirGlo-CALB2-3'UTR delta ARE

Plasmid

#74425

Purpose

Calb2 UTR with mutated AU binding sequence

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

Insert

CALB2 3'UTR (CALB2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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