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Showing: 7701 - 7750 of 148051 results

  1. p5E--3mnx1

    Plasmid
    #74632
    Purpose
    Can be used to drive expression specifically in zebrafish motor neurons
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    motor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Tags
    Expression
    Mutation
    Promoter
    -3mnx1
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    74632-plasmid-map-sequence-id-207910

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  2. CRY2-INPP5E

    Plasmid
    #79562
    Purpose
    expression of CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif
    Depositor
    Olof Idevall-Hagren, Pietro De Camilli
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    INPP5E (INPP5E Human)
    Use
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    C-terminal region, aa 214_644, C641A mutation to …
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. mCherry-Keratin-17

    Plasmid
    #55065
    Purpose
    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Keratin (KRT18 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    V282L
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pLM-AMA18.0 dCas9-VPR

    Plasmid
    #138945
    Purpose
    Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Expression
    Mutation
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138945-plasmid-map-sequence-id-366628

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  11. TFORF1808

    Plasmid
    #143849
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TBX18 (TBX18 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143849-plasmid-map-sequence-id-391577

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  12. pSLC-246

    Plasmid
    #73190
    Purpose
    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
    Depositor
    Swaine Chen
    Article
    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8
    Insert
    PrhaB-tse2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaB
    Available since
    July 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73190-plasmid-map-sequence-id-151367

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  21. pAAV-EF1a-DIO-hM3D(Gq)-mCherry

    Plasmid
    #50460
    Purpose
    Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoter
    Depositor
    Bryan Roth
    Insert
    hM3D(Gq)-mCherry (CHRM3 Human)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    EF1a
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50460-plasmid-map-sequence-id-182307

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  32. AICSDP-57: SMC1A-mEGFP

    Plasmid
    #114406
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SMC1A
    Depositor
    Allen Institute for Cell Science
    Insert
    SMC1A Homology Arms with linker-mEGFP (SMC1A Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114406-plasmid-map-sequence-id-220833

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  34. CXCR2-DuET

    Plasmid
    #213219
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR2 (CXCR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213219-plasmid-map-sequence-id-430781

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  36. pSCM001

    Plasmid
    #124605
    Purpose
    Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH
    Depositor
    David Summers
    Article
    Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1
    Insert
    mCherry-pHluorin
    Use
    Tags
    mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…
    Expression
    Bacterial
    Mutation
    Promoter
    pDAB
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    124605-plasmid-map-sequence-id-242761

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  38. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid
    #183409
    Purpose
    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183409-plasmid-map-sequence-id-361258

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  46. SPLICSFAST-ER-MT-P2A

    Plasmid
    #214892
    Purpose
    Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-ER-MT-P2A
    Use
    Tags
    none
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214892-plasmid-map-sequence-id-423478

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Showing: 7701 - 7750 of 148051 results