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AAV-U6gRNA1-U6gRNA2-TnT-Cre
Plasmid#87682PurposeAAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.DepositorInsertsgRNA1
gRNA2
Cre
UseAAVTagsExpressionMutationPromoterU6 and cTnTAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-mDyn3 pcDNA3
Plasmid#36265DepositorInsertDynamin 3 (Dnm3 Mouse)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG_smFP V5
Plasmid#59758Purposeencodes multiple copies of an epitope tag V5DepositorInsertsmFP _V5
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Cas9_puro
Plasmid#108100PurposeLentiviral expression plasmid of spCas9 with puromycin resistance geneDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
N1-p18-EGFP
Plasmid#42334DepositorInsertp18 (LAMTOR1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-KD LKB1
Plasmid#8591DepositorInsertLKB1 (STK11 Human)
UseTagsFLAGExpressionMammalianMutationK78I: lysine 78 mutated to isoleucine; kinase deadPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Blast
Plasmid#118055PurposeUsed for expression of sgRNA in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-csGBP
Plasmid#108875PurposeExpression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)DepositorInsertmKate2-P2A-APEX2-csGBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA
Plasmid#55202PurposePlasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.DepositorInsertdsRed2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC189
Plasmid#32114DepositorTypeEmpty backboneUseTransposon delivery vectorTags3x FLAGExpressionMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_erGAP3
Plasmid#78118PurposeLow affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulumDepositorInserterGAP3
UseTagsKDEL and calreticulin signal peptideExpressionMammalianMutationS175G, D180Y, I167V and L15Q in GFP moiety and D…PromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMetYC-Dest
Plasmid#105081PurposeYeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.DepositorTypeEmpty backboneUseTagsCub-PLVExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Kinesin11-N-18
Plasmid#54137PurposeLocalization: Motor Protein/Microtubule Filaments, Excitation: 487, Emission: 509DepositorInsertKinesin11 (KIF11 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBRPyCAG-Mettl3-dsRed-IRES-puro
Plasmid#61516PurposeConstitutive expression of murine wild-type Mettl3DepositorInsertMettl3 (Mettl3 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGGAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-U6-empty
Plasmid#140580PurposeExpresses a gRNA and mCherry in mammalian cellsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
CIP4-pmCherryC1
Plasmid#27685DepositorInsertCIP4 (Trip10 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAS
Plasmid#60847PurposeExpresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeastDepositorInsertsS. pyogenese Cas9
RNA pol III promoter (tRNA-Tyr)
hepatitis delta virus ribozyme, genomic
sgRNA
UseCRISPR and Synthetic BiologyTagsNLS/His8 and TTT 3' extension prior to sgRNAExpressionBacterial and YeastMutationL4 is UUCG tetraloop and guide targets LYP1 (CATA…PromoterAvailable sinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDest-565
Plasmid#11520PurposeGateway destination vector for bacterial expression; His,GST tagDepositorTypeEmpty backboneUseGateway destination vectorTagsHis6-GSTExpressionBacterialMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG424GAL-ccdB
Plasmid#14151DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only
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