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Plasmid#85707PurposeBackbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only
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LV anti-FITC-LaG16-sNRR-T2A-BFP
Plasmid#194930PurposeLV construct encoding sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV mTurquoise2-shadowG tandem
Plasmid#157774PurposeProduces a fusion between mTurquoise2 and shadowG. It can be used as a positive control for FRET (using FLIM)DepositorTypeEmpty backboneUseTagsmTurquoise2 and shadowGExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-TPT1-N-10
Plasmid#54284PurposeLocalization: Microtubule Associated Protein, Excitation: 487, Emission: 509DepositorInsertTPT1 (TPT1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
SIN40C.SFFV.dTomato.miR30n
Plasmid#169277PurposeConstitutive overexpression of miRNA or shRNA with dTomato as reporter fluorescent proteinDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC22A16
Plasmid#132023PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC22A16 (SLC22A16 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pISH-NQO1
Plasmid#136766PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH) for Nqo1DepositorInsertNqo1 (Nqo1 Mouse)
UseIn situ probeTagsExpressionMutationISH Probe insert, V184A- Please see depositor co…PromoterAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pM-Cas9
Plasmid#196325Purpose35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GPDepositorInsertFull length TSWV M antigenome encoding SpCas9 in place of viral GP
UseCRISPRTags3×flagExpressionPlantMutationSee depositor comments belowPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI H2B-GFP
Plasmid#92399PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.DepositorInsertIRES H2B EGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK 3'FBXW7 UTR
Plasmid#26649DepositorInsertFBXW7 (FBXW7 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationContains only the 3'UTR of FBXW7, nucleotide…PromoterAvailable sinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRG659_lexO-FseI_LexA-TF_LEU2MX
Plasmid#154816PurposeInducible expression of FseI endonuclease in S. cerevisiaeDepositorInsertsFseI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9n(D10A)-Blast
Plasmid#63593PurposeExpresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertsCas9
Blasticidin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationD10A, nickase mutantPromoterEFS-NSAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLPCX roGFP2-Orp1
Plasmid#64991PurposeMammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)DepositorInsertOrp1 (HYR1 Budding Yeast)
UseRetroviralTagsroGFP2ExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
VARP GFP-pLXIN
Plasmid#62950Purposefull length human VARP with C-terminal EGFP tag cloned into pLXINDepositorInsertVARP (ANKRD27 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationsilent mutations of amino acids 422-428 to produc…PromoterLTRAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-StrepII-TagRFP-CohI
Plasmid#58710PurposeDetection construct to label dockerin type I containing fusion proteinsDepositorInsertTagRFP-CohI
UseTagsStrepIIExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBMN mCherry-OPTN(F178A/D474N)
Plasmid#119684PurposeFor mammalian expression of LIR/UBD double mutant OPTNDepositorInsertOPTN (OPTN Human)
UseRetroviralTagsmCherryExpressionMammalianMutationF178A/D474NPromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA
Plasmid#171383PurposeExpression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.DepositorInserthumanized minidCas13X.1-REPAIRv2
UseTagsHAExpressionMammalianMutationPromoterCMV, U6Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.3_SOX2
Plasmid#26817DepositorInsert5'UTR-SOX2-3'UTR (SOX2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5FRT/TO-SVIP-Strep-HA
Plasmid#113491PurposeExpression of human SVIP with C-terminal strep-HA tagDepositorInsertSVIP (SVIP Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA
Plasmid#140222PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPRDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
UseTagsmCherryExpressionMammalianMutationD832APromoterPGKAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE hygro MEN1 WT
Plasmid#11024DepositorInsertMEN1 (MEN1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RVR
Plasmid#108861PurposeLbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutationsDepositorInsertLbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationG548R, K554V and Y558R, Cpf1 is rice codon optimi…PromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Progerin-CS
Plasmid#69064Purposeencodes Progerin with a substitution of cysteine in CaaX-motif by a serineDepositorInsertProgerin (LMNA Human)
UseRetroviralTagsExpressionMammalianMutationProgerin with substitution of cysteine in CaaX-mo…PromoterCMVAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB-Halo,IRES-eGFP
Plasmid#164519PurposeAll-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-NPM1wt-C1
Plasmid#131818PurposeExpresses HcRed-NPM1wildtype (human) in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
UseTagsHcRedExpressionMammalianMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXP418
Plasmid#26843DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
AcGFP-MSH3
Plasmid#215122PurposeExpresses AcGFP-MSH3 in mammalian cells from a retroviral vector.DepositorInsertMSH3 (MSH3 Human)
UseRetroviralTagsAcGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
mVenus
Plasmid#198192PurposeSpectral correction: Expression of mVenusDepositorInsertmVenus
UseTagsExpressionMammalianMutationA206KPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
StrepII-tag
Plasmid#55181PurposeStreptactin-tagDepositorInsertStrepII tag
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ140
Plasmid#99895PurposeEmpty vector with attL5-attL2 recombination sites for Gateway CloningDepositorTypeEmpty backboneUseCRISPR and TALENTagsExpressionMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
HLTV-hTRIM21
Plasmid#104973PurposePurification of His-Lipoyl-hTRIM21 protein from bacteriaDepositorInsertTripartite Motif containing 21 (TRIM21 Human)
UseTagsHis-LipoylExpressionBacterialMutationPromoterT7Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-ybbR-HIS-Xyl-DocI
Plasmid#58711PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertXyl-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationchanged Threonin 120 to Cysteine in Xylanase Doma…PromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET42b-ABE7.10
Plasmid#120398PurposeExpresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorInsertABE7.10
UseTagsHisExpressionBacterialMutationMammalian codon-optimizedPromoterT7Available sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
MS2_GFP
Plasmid#61764PurposeExpresses MS2 coat protein fused to GFPDepositorInsertMS2 coat protein fused to eGFP
UseLentiviralTagseGFPExpressionMammalianMutationPromoterUbc promoterAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
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ER-mGreenLantern
Plasmid#164463PurposeLocalization and retention of mGreenLantern fluorescent protein in the ERDepositorInsertmGreenLantern
UseTagsCalreticulin and KDELExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
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lentiGuide-mMaroon
Plasmid#217008Purposelentiviral plasmid for gRNA cloning with selectable mMaroon expressionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-STX17TMΔC
Plasmid#221737PurposeStable expression of GFP-STX17TMΔC in mammalian cellsDepositorInsertSTX17 (STX17 Human)
UseRetroviralTagsGFPExpressionMammalianMutationdeleted amino acids 1-228 and 227-302PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
CD55-full length
Plasmid#220874PurposeExpresses CD55 variant in mammalian cellsDepositorInsertCD55 (CD55 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMWAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG
Plasmid#218163PurposeThis plasmid harbors the base editor eSCBE3-NG along with an sgRNA cloning cassette, facilitating high-efficiency cytosine base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsescbe3-NG
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionTagsExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG-HF1
Plasmid#218164PurposeThis plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsescbe3-NG-HF1
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionTagsExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
peGFP C3-SPG20-PAAA
Plasmid#218581PurposeExpresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCHDepositorInsertSPG20 (SPART Human)
UseTagseGFPExpressionMammalianMutationchanged Proline 172 to Alanine and Tyrosine 174 t…PromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
peGFP C1-SPG20 SC domain
Plasmid#218580PurposeExpresses GFP-tagged SC domain of human SPG20 in mammalian cellsDepositorInsertSPG20 (SPART Human)
UseTagseGFPExpressionMammalianMutationdeleted amino acids 1-417PromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDNR-mCherry-T2A-3xPuroR-Frame 1
Plasmid#206992PurposeDonor plasmid for Frame 1 with mCherry tag and triple Puromycin resistant marker (3xPuroR)DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDNR-mCherry-T2A-3xPuroR-Frame 2
Plasmid#206993PurposeDonor plasmid for Frame 2 with mCherry tag and triple Puromycin resistant marker (3xPuroR)DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAS
Plasmid#206994PurposePlasmid expressing both SpCas9 and SaCas9DepositorUseTagsP2AExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDNR-gRNA
Plasmid#206990PurposeA plasmid for expression of SaCas9-sgRNA targeting donor plasmidsDepositorInsertDonor plasmid-targeting SaCas9-gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only