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pYFP
Plasmid#175592PurposeTet inducible, KAN Resistant, mVenus gene on a low copy backbone. Used as a negative control to the pRelA_YFP plasmid.DepositorInsertmVenus
UseTagsExpressionBacterialMutationAn improved monomeric YFP. See doi: 10.1021/bi051ā¦PromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human-Dysferlin-Original
Plasmid#60216PurposeHuman dysferlin (accession #NM_003494) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogenās gateway system.DepositorInsertDysferlin (DYSF Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRRlsinPGK_H2BGFP_WPRE
Plasmid#91788PurposeConstitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.DepositorInsertHuman histone H2B (H2BC11 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterPGKAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab27A
Plasmid#89237PurposeExpression of GFP-tagged human Rab27A in mammalian cellsDepositorInserthuman Rab27A (RAB7A Human)
UseTagseGFPExpressionMammalianMutationwild typePromoterCMVAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE16
Plasmid#90357PurposeCREB - Cyclic AMP gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterCREBAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.tRFP
Plasmid#57819PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
PSD-95-pTagRFP
Plasmid#52671Purposeexpression of TagRFP-tagged PSD-95DepositorInsertPSD-95 (Dlg4 Rat)
UseTagstagRFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pERB264
Plasmid#67764PurposeTags peroxisomes with GFP and HalotagDepositorInsertPEX3-GFP-Halo
UseTagsGFP and HalotagExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC0078 RESCUE
Plasmid#130661PurposepCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/DepositorInsertdRanCas13b-ADAR2dd (RESCUE)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
S96 ELP
Plasmid#68393PurposeElastin-like polypeptide (VPGxG) with 96 repeats of Serine guest residueDepositorInsertS96 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4 HisMax-V5-GFP-RRBP1
Plasmid#92150PurposeExpresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.DepositorInsertRRBP1 (RRBP1 Human)
UseTagsCalreticulin signal peptide, EGFP (A207K mutationā¦ExpressionMammalianMutationIn RRBP1: N951S and T1329L. E1279 is also missing.PromoterCMVAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag
Plasmid#209652PurposeExpression of iATPSnFR2, medium affinityDepositorInsertiATPSnFR2.A95K.HaloTag
UseAAVTagsExpressionMutationA95KPromoterCAGAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE34
Plasmid#90376PurposeMAPK - Elk1 gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterELK1Available sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
PVS10
Plasmid#104398Purposepolycistronic vector for expression of E. coli core RNAPDepositorUseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
N114 nLV EF-1a-Gal4-VP16-PGK-Puro
Plasmid#44014DepositorInsertGal4/DBD (1-147)
UseLentiviralTagsVP16/Activation/DomainExpressionMutationPromoterEF-1aAvailable sinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-LAMP1-RFP-3xHA
Plasmid#102932PurposeExpress a lysosomal TagDepositorInsertLAMP1
UseLentiviralTagsRFP-HAExpressionMutationPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE4
Plasmid#90338PurposeNRF2 - Antioxidant response element gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterNRF2Available sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLGB30
Plasmid#126620PurposeSuicide vector for allelic replacement in Bacteroides, tetracycline selection and rhamnose-inducible ss-Bfe1 counterselectionDepositorTypeEmpty backboneUseAllellic exchange in bacteriaTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
EMTB-mCherry
Plasmid#26742DepositorInsertensconsin (MAP7 Human)
UseProbe for microtubulesTags2x mCherry and MycExpressionMammalianMutationMicrotubule binding-domain of ensconsin (amino acā¦PromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe4_GCaMP6f
Plasmid#213939PurposeAAV construct expressing GCaMP6f driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_GCaMP6fDepositorInsertGCaMP6f
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-pBAD
Plasmid#54762PurposeLocalization: Protein Expression Vector , Excitation: 488, Emission: 507DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis and EGFPExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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mOrange2-pBAD
Plasmid#54531PurposeLocalization: Protein Expression Vector , Excitation: 549, Emission: 565DepositorTypeEmpty backboneUseTags6xHis and mOrange2ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRDB_345
Plasmid#231113PurposeABE activity-based selection cloning vectorDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDB_347
Plasmid#231114PurposeCBE activity-based selection cloning vectorDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Neo DEST (705-1)
Plasmid#17392Purpose3rd gen lentiviral Gateway destination vector, expression, CMV promoter, NeoDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-E01DARPin
Plasmid#198287PurposeProduces PVCs retargeted with anti-EGFR DARPin E01DepositorInsertPVCpnf_pvc13-E01DARPin
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GĪ±i*-BERKY3
Plasmid#158198PurposeBRET biosensor for detection of GĪ±i-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-Scrambled
Plasmid#136035PurposeScrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)DepositorInsertNone (Scrambled)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIP-Flag-TfebAA
Plasmid#79014PurposeFor mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.DepositorInsertTfeb (Tfeb Mouse)
UseTagsFlagExpressionMammalianMutationS142A, S211APromoterpCAGGsAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AktAR2
Plasmid#184047PurposeImproved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.DepositorInsertExRai-AktAR2
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG PSAM4 GlyR IRES EGFP
Plasmid#119739PurposeChemogenetic inhibitor expression plasmidDepositorInsertPSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
UseTagsExpressionMammalianMutationL131G, Q139L, Y217FPromoterCAGAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-GFP(LaG2) PAGER(TF)
Plasmid#229996PurposeExpresses anti-GFP PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-LaG2-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_GR-RE_MLPmin_BC1092-luc2
Plasmid#227112PurposeGlucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092DepositorInsert12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pREP-8xARE-GFP-SV40-BFP
Plasmid#134910PurposeNRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFPDepositorInserts8xARE- minimal promoter - GFP
TagBFP
UseTagsExpressionMammalianMutationPromoter8xARE - minimal promoter and SV40Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-LUC2CP/ARE
Plasmid#62857PurposeSplicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseTagsExpressionMammalianMutationdestabilizing sequences (DS) added to the C termiā¦PromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)
Plasmid#209078PurposeNgn2 mediated cortical neuron induction, along with SNCA(3K) over-expressionDepositorUseTagssfGFPExpressionMammalianMutation3K mutant: E35K, E46K, E61KPromoterTRE4GAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2pA2-ubb:secHsa.ANNEXINV-mVenus, cryaa:RFP
Plasmid#92388PurposeCell death reporterDepositorInsertANNEXINV (ANXA5 Human)
UseZebrafish expressionTagsmVenusExpressionMutationPromoterubbAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only
