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Addgene

pLGB30
(Plasmid #126620)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126620 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    new backbone
  • Modifications to backbone
    NEBuilder assembly reaction to fuse: a) rhamnose promoter, amplified from gene BT_3763 from B. thetaiotaomicron VPI-5482 (this promoter drives ss-bfe1 expression) b) ss-bfe1 gene and multiple cloning site, amplified from pLGB13, using oLGB110 and oLGB120 c) tetracycline resistance casette, amplified from vector pYT646b (Tang and Malamy, 2000), using oLGB121 and oLGB122 d) origin of transfer and replication, amplified from pLGB13 e) RhaR cassette (BT_3768), amplified from B.thetaiotaomicron VPI-5482
  • Vector type
    allellic exchange in bacteria
  • Selectable markers
    tetracycline (Bacteroidales)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    S17-lambda pir
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLGB30 was a gift from Laurie Comstock (Addgene plasmid # 126620 ; http://n2t.net/addgene:126620 ; RRID:Addgene_126620)
  • For your References section:

    Streamlined Genetic Manipulation of Diverse Bacteroides and Parabacteroides Isolates from the Human Gut Microbiota. Garcia-Bayona L, Comstock LE. mBio. 2019 Aug 13;10(4). pii: mBio.01762-19. doi: 10.1128/mBio.01762-19. 10.1128/mBio.01762-19 PubMed 31409684