Showing: 3041 - 3060 of 3839 results
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Plasmid#103870PurposeHigh specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.DepositorInsertsUseCRISPRTagsGSGGGGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…PromoterAvailabilityAcademic Institutions and Nonprofits only
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pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090
Plasmid#103871PurposedPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAVDepositorInsertsUseCRISPRTagsGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…PromoterAvailabilityAcademic Institutions and Nonprofits only -
PCS2+Cas9-mSA
Plasmid#103882PurposeCas9 mSA for in vitro transcription for gene editing in Mouse EmbryosDepositorInsertCAS9-MSA
UseCRISPRTagsMSA (monomeric streptavidinExpressionMutationnonePromoterSP6AvailabilityAcademic Institutions and Nonprofits only -
pDG02583
Plasmid#104129PurposeOptimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)DepositorInsertNEDD8-specific protease 1 (NEDP1)
UseTags14x Histidine tag, Maltose binding protein, and S…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTP943
Plasmid#104157PurposeBacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)DepositorInsertAnti-mouse IgG1 Fab specific nanobody TP886
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTP1112
Plasmid#104158PurposeBacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)DepositorInsertAnti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTP1122
Plasmid#104159PurposeBacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)DepositorInsertAnti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTP1005
Plasmid#104160PurposeBacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)DepositorInsertAnti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTP1172
Plasmid#104161PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTP1183
Plasmid#104163PurposeBacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)DepositorInsertAnti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTP955
Plasmid#104164PurposeBacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)DepositorInsertAnti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO-sgRNA-puro
Plasmid#104321Purpose3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1/TOAvailabilityAcademic Institutions and Nonprofits only -
pAW12.lentiguide.GFP
Plasmid#104374PurposeGFP vector for cloning in guidesDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET259-pUC57 24xMS2V6
Plasmid#104391PurposeMS2V6 cloningDepositorInsert24xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lwa_Cas13a_WT
Plasmid#91865PurposeBacterial expression for Cas13aDepositorInsertLwaCas13a
UseCRISPRTagsHis6-MBP-TEV siteExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pC014 - LwCas13a-msfGFP
Plasmid#91902PurposeExpresses active LwCas13a for mammalian RNA targetingDepositorInsertLwCas13a
UseCRISPRTagsNLS and msfGFP-NLS-3xHAExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pC015 - dLwCas13a-NF
Plasmid#91905PurposeExpresses negative-feedback dLwCas13a for mammalian RNA targeting/imagingDepositorInsertdLwCas13a
UseCRISPRTagsNLS and eGFP, ZF CCR5TC, KRAB-A, NLSExpressionMammalianMutationR474A and R1046APromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pC016 - LwCas13a guide expression backbone with U6 promoter
Plasmid#91906PurposeBackbone for cloning LwCas13a guides under U6 promoterDepositorHas ServiceCloning Grade DNAInsertLwCas13a Guide site
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pC017 - LwCas13a guide expression backbone with tRNAval promoter
Plasmid#91907PurposeBackbone for cloning LwCas13a guides under tRNAval promoterDepositorInsertLwCas13a Guide site
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pC019 - LwCas13a from Leptotrichia wadei
Plasmid#91909PurposeExpresses LwCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLwCas13a and guide expression cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits
Showing: 3041 - 3060 of 3839 results