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Plasmid#19885DepositorInsertTrinucleotide repeat containing 6C (TNRC6C Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-E1799K
Plasmid#69009Purposeactivating MTOR mutationDepositorInsertMTOR-E1799K (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Glutamate 1799 to LysinePromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 WT MYOC eGLuc2 FLAG
Plasmid#192820PurposeExpresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tagDepositorInsertmyocilin (MYOC Human)
UseTagsFLAG and enhanced Gaussia luciferase (M43I/M110I)ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
hMLKL NB(1-140)-2xFv-flag
Plasmid#106074Purposeexpression in mammalian cellsDepositorInserthuman MLKL NB(1-140)-2xFv-flag (MLKL Human)
UseRetroviral; Tet-on retrovirusTagsflagExpressionMammalianMutationamino acids 1-140PromoterDox-inducibleAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Flag hMcl-1(S159A)
Plasmid#25372DepositorInserthMCL1(S159A) (MCL1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationchanged serine 159 to alaninePromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-379 FLAG UnaG
Plasmid#130131PurposePPI (BiFC/TriFC) assay vectorDepositorInsertUnaG
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2 (T1462A)
Plasmid#14130DepositorInsertTSC2 T1462A (TSC2 Human)
UseTagsFlagExpressionMammalianMutationT1462APromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/AUG-FFLuc-3XFLAG
Plasmid#127333PurposeExpress 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codonDepositorInsertFirefly luciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCV40Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-MIOS(1-875) in pRK5
Plasmid#184561PurposeCMV-driven expression of an N-terminally Flag-tagged MIOS (core subunit of GATOR2) — native sequence for transient expression in mammalian cells.DepositorInsertMIOS (MIOS Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV10-3xFlag Skp2 delta-F
Plasmid#81116Purposemammalian expression of F-box deleted Skp2DepositorInsertSKP2 (SKP2 Human)
UseTags3xFlagExpressionMammalianMutationdeleted F boxPromoterCMVAvailable sinceSept. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-MAP1LC3BK51A
Plasmid#175754PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3B K51ADepositorInsertMAP1LC3B (MAP1LC3B Human)
UseLentiviralTagsExpressionMutationK51APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MTS-KD-c-Src-FLAG
Plasmid#44653DepositorInsertv-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
UseTagsFLAG tag and mitochondria-targeting sequence (MTS…ExpressionMammalianMutationchanged lysine 298 to methioninePromoterCMV promoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GFP-Flag-UTR
Plasmid#183235PurposeTo express FLAG tagged eGFPDepositorInsertEGFP
UseTagsFLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-Flag-Mili-EGFP
Plasmid#212576PurposeLentiviral expression of Flag-tagged mouse Mili in mammalian cellsDepositorInsertMili (Piwil2 Mouse)
UseLentiviralTags5x Flag and SNAP tagExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAmCherry-FLAG:cat
Plasmid#211832PurposePlasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.DepositorInsertPAmCherry-FLAG-cat
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-BirA-FLAG
Plasmid#105786PurposeExpression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-BirA-FLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sa-dCas9-NLS-3xFLAG/pcDNA3.1
Plasmid#98041PurposeExpresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interestDepositorInsertSa-dCas9-NLS-3xFLAG
UseCRISPRTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianMutationD10A + H557APromoterCMVAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3B G120A
Plasmid#123093PurposeExpresses 3xFLAG-LC3B G120A in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTags3xFLAGExpressionMammalianMutationGlycine 120 to AlaninePromoterCMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-EDF1
Plasmid#38079DepositorInsertEDF1 endothelial differentiation-related factor 1 (EDF1 Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIPK3[S227D]-FLAG
Plasmid#199320Purposemutated phosphorylation site (MLKL interaction permanent active)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
UseTagsFLAGExpressionMammalianMutationchanged Serine 227 to Aspartic AcidPromoterAvailable sinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only