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pSPgRNA

Plasmid

#47108

Purpose

Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

SPgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pSOUP

Plasmid

#165419

Purpose

The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

Depositor

Wendy Harwood, John Innes Centre - Crop Transformation and Genome Editing Group

Article

Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

Insert

Rep A

Use

Helper plasmid required for replication for all p…

Tags

Expression

Mutation

Promoter

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRDA_550

Plasmid

#203398

Purpose

All-in-one lentiviral expression of gRNA and EnAsCas12a

Depositor

John Doench

Article

Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

Insert

Enhanced Acidaminococcus Cas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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3XFlag-pA-Tn5-Fl

Plasmid

#124601

Purpose

Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

Depositor

Steven Henikoff

Article

Kaya-Okur et al Nat Commun. 2019 Apr 29;10(1):1930. doi:

Insert

Protein A and hyperactive Tn5 transposase (Tnp) fusion protein

Use

Tags

Protein A and Tn5 transposase fusion protein with…

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTarget_CAST

Plasmid

#127926

Purpose

Target plasmid containing PSP1

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

PSP1 target (GGTT PAM)

Use

Tags

Expression

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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IFN-Beta_pGL3

Plasmid

#102597

Purpose

IFN-beta promoter driving luciferase. Use in IFN reporter assay.

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

Human IFN-Beta promoter (IFNB1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-ARP2-N-14

Plasmid

#54980

Purpose

Localization: Actin Related Protein, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

ARP2 (ACTR2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pMCSG53_N

Plasmid

#167255

Purpose

Bacterial expression of Nucleocapsid protein (residues 1-419)

Depositor

Alexei Savchenko

Insert

N protein (residues 1-419) (N SARS-CoV-2)

Use

Tags

His6-TEV site

Expression

Bacterial

Mutation

none

Promoter

Available since

April 27, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CAG-BFP-sesRNA-GFP-WPRE-pA

Plasmid

#192063

Purpose

Expression of BFP, sesRNA in mammalian cells, with GFP as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for tdTom

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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scAAV-hIBA1a-GFP

Plasmid

#214146

Purpose

Self-complementary AAV vector packaging GFP under the human Iba1 promoter

Depositor

Chun-Li Zhang

Article

Serrano et al bioRxiv 2023.12.12.571321

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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