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Plasmid#86461PurposeA modified version of the Promega pGL4.23 vector. Contains the MYC promoter in place of the minimal promoter and polyadenylation signals flanking the enhancer cloning site.DepositorTypeEmpty backboneUseLuciferaseTagsExpressionMammalianMutationPromoterMYCAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only
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pMSSM16: pCMV-HA-NLS-SpuFz1
Plasmid#205271PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1DepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ centrin-mKate2
Plasmid#105941Purposein vitro transcription, zf centrin N-terminally tagged with mkate2DepositorInsertCentrin
UseTagsmKate2ExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSCL.140
Plasmid#184995PurposeExpress Cas9 in human cellsDepositorInsertSpCas9
UseTagsSV40NLSExpressionMammalianMutationPromoterTetOn-3gAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ef1a-DIO-eGCaMP(2+)
Plasmid#201150PurposeAAV virus production for Cre recombinase dependent expression of eGCaMP(2+)DepositorInserteGCaMP_2+
UseAAV, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUNIV-EGFP
Plasmid#24706DepositorInsertEGFP (eGFP Aequoria victoria)
UseTagsExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceDec. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-PRKAA1ΔC
Plasmid#30306DepositorInsertAMP-activated Protein Kinase α1 ΔC (Prkaa1 Rat)
UseTagsGFPExpressionMammalianMutationdeleted amino acids Pro 526 to Gln 548PromoterAvailable sinceAug. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pX330S-5
Plasmid#58781PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRC319
Plasmid#61272PurposeAlso referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Notch1 ICv-6MT
Plasmid#41730DepositorInsertNotch-1 Intracellular Domain (Notch1 Mouse)
UseTags6xMycExpressionMammalianMutationContains murine Notch-1 protein beginning at Vali…PromoterAvailable sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x4
Plasmid#58768PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HIRA-polyA
Plasmid#59781PurposeExpress H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertHIRA
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV0279
Plasmid#149688PurposeBacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTagDepositorInsertSUMO_Eu1-MBP
UseTags3xFLAG-tag and His14-tagExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-MYC-e3
Plasmid#120341PurposepGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.DepositorInsertchr8:129056967-129058438 (hg19)
UseLuciferaseTagsExpressionMammalianMutationPromoterMYCAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iG2-wPRE-pA
Plasmid#60489PurposeeGFP-expressing lentiviral vector with IRES and pA-signal; suitable for use as NRTLV (non-reverse transcribable lentiviral vector)DepositorInsertIRES-eGFP; wPRE; BGH-pA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
Membrane mCherry
Plasmid#210470PurposePlasma membrane localizing mCherry using Human LCK proto-oncogene sequenceDepositorInsertLCK membrane mCherry (LCK Synthetic, A.victoria, Human)
UseSynthetic Biology; Sea urchin, mammal, zebrafishTagsmCherryExpressionMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ106: pRS426-His-MBP-NlovFz2_Native
Plasmid#205253PurposeS. cerevisiae expression vector for NlovFz2 native guideDepositorInsertNlovFz2
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ45: pRS426-His-MBP-SpuFz1_Native
Plasmid#205252PurposeS. cerevisiae expression vector for SpuFz1 native guideDepositorInsertSpuFz1
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH625_pJH_LldR_PlldR-sfGFP.0TAG
Plasmid#163724PurposeLactate-inducible expression of green fluorescent protein encoding zero amber codonsDepositorInsertlldr gfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB8_4-3i-ATP8_v8_AGKS
Plasmid#191513PurposeExpression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Right)DepositorInsertv8 ZF-DdCBE 4-3i-ATP8 AGKS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only