Addgene

We narrowed to 7,655 results for: mel

Showing: 281 - 300 of 7655 results

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HA-doraWT_rescue_construct

Plasmid

#190608

Purpose

Puromycin-selectable expression of HA-tagged Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Use

Tags

3xHA

Expression

Insect

Mutation

Promoter

Actin-5c

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMCh-76

Plasmid

#92288

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cells

Depositor

Roy Parker, Marina Chekulaeva

Article

Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

Insert

dGW182 CED (gw Fly)

Use

Tags

NHA

Expression

Insect

Mutation

dGW182 CED: aa 940-1215

Promoter

Ac5

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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D-raf G621S

Plasmid

#39769

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Use

Tags

Expression

Insect

Mutation

G621S

Promoter

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf C295S

Plasmid

#39768

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Use

Tags

Expression

Insect

Mutation

C295S

Promoter

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf F290I

Plasmid

#39766

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Use

Tags

Expression

Insect

Mutation

F290I

Promoter

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf R217L+S620A

Plasmid

#39765

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Use

Tags

Expression

Insect

Mutation

R217L and S620A

Promoter

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf R217L+C295S

Plasmid

#39764

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Use

Tags

Expression

Insect

Mutation

R217L and C295S

Promoter

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
KHBD00749

Plasmid

#39755

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG15455 (RunxB Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00632

Plasmid

#39702

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG34422 (CG34422 Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
Renilla luciferase-Pol III

Plasmid

#37380

Depositor

Norbert Perrimon

Article

Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

Insert

PolIII-Renilla control reporter (RpIII128 Fly)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

RNA PolIII 128 subunit (RpIII128)

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1 IntS8 AA 1-308

Plasmid

#196905

Purpose

Expresses His-tagged Drosophila IntS8 amino acids 1-308

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator subunit 8 (IntS8 Fly)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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YFP Rab4DN

Plasmid

#99019

Purpose

Localization and visualization of the dominant negative form of Rab4

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

Rab4 DN (Rab4 Fly, Synthetic)

Use

Tags

YFP

Expression

Mammalian

Mutation

S22N Dominant negative

Promoter

CMV

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST-SIK3 T196E

Plasmid

#52975

Purpose

insect expression of SIK3 T196E constitutively active mutant

Depositor

Biao Wang, Marc Montminy

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Use

Tags

HA

Expression

Insect

Mutation

T196E constitutively active mutant

Promoter

Hsp70

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
pUAST-SIK3 T196A

Plasmid

#52974

Purpose

insect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)

Depositor

Biao Wang, Marc Montminy

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Use

Tags

HA

Expression

Insect

Mutation

T196A LKB1 phosphorylation defective mutant

Promoter

Hsp70

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only
pUAST-SIK3 K70M

Plasmid

#52973

Purpose

insect expression of SIK3 K70M (kinase dead mutant)

Depositor

Biao Wang, Marc Montminy

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Use

Tags

HA

Expression

Insect

Mutation

K70M kinase dead mutant

Promoter

Hsp70

Available since

May 5, 2014

Availability

Academic Institutions and Nonprofits only
pUASt-SIK3 S563A

Plasmid

#52976

Purpose

insect expression of SIK3 S563A PKA phosphorylation defective mutant

Depositor

Biao Wang, Marc Montminy

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Use

Tags

HA

Expression

Insect

Mutation

S563A PKA phosphorylation defective

Promoter

Hsp70

Available since

May 5, 2014

Availability

Academic Institutions and Nonprofits only
pUASp-Flag-Myc-Qin-E3-attB

Plasmid

#44209

Depositor

Phillip Zamore

Article

Zhang et al Mol Cell. 2011 Nov 18;44(4):572-84. doi:

Insert

Qin (qin Fly)

Use

Fly transformation

Tags

3xFlag and 6xMyc

Expression

Mutation

lacking 5xTudor domains on C-term; aa1-274 only

Promoter

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
KHBD00390

Plasmid

#34502

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6121 (Tip60 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pAc5.1B-lambdaN-HA-DmNot1_1467-1717_AE

Plasmid

#148598

Purpose

Insect Expression of DmNot1_1467-1717

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmNot1_1467-1717 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_J

Plasmid

#146710

Purpose

Insect Expression of DmNot1

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmNot1 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

11 silent mutations compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge