pRSFDuet-1 IntS8 AA 1-308
(Plasmid #196905)

• Purpose: Expresses His-tagged Drosophila IntS8 amino acids 1-308
• Depositing Lab: Jeremy Wilusz
• Publication: Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)00902-4. doi: 10.1016/j.molcel.2023.10.035.

Backbone

• Vector backbone: pRSF-Duet-1
• Total vector size (bp): 4726
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Integrator subunit 8
• Species: D. melanogaster (fly)
• Entrez Gene: IntS8 (a.k.a. Dmel_CG5859, CG5859, Dmel\CG5859, EG:EG0003.5, Int8, intS8)
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: GGGGAATTGTGAGCGGATAACAATT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry