D-raf R217L+C295S
(Plasmid
#39764)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 39764 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCaSpeR-4
- Backbone size w/o insert (bp) 7855
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert named-raf
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SpeciesD. melanogaster (fly)
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MutationR217L and C295S
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Entrez GeneRaf (a.k.a. Dmel_CG2845, 11-29, C110, CG2845, D-RAF, D-Raf, D-raf, D-raf1, DRaf, DRaf1, Dmel\CG2845, Draf, Draf-1, Draf1, EG:BACH48C10.3, Phl, RAF, Raf/phl, Raf1, dRaf, draf, l(1)2Fe, l(1)G0475, l(1)ph, l(1)phl, l(1)pole hole, l(1)polehole, l(1)polehole/draf, l(1)raf, lincRNA.937, ph, ph1, phl, raf, raf-1, raf1, rafl)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pCaspeR4-5
- 3′ sequencing primer pCaspeR4-3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Perrimon Lab plasmid #88
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
D-raf R217L+C295S was a gift from Norbert Perrimon (Addgene plasmid # 39764 ; http://n2t.net/addgene:39764 ; RRID:Addgene_39764) -
For your References section:
Genetic and molecular analyses of mutations involved in Drosophila raf signal transduction. Lu X, Melnick MB, Hsu JC, Perrimon N. EMBO J. 1994 Jun 1;13(11):2592-9. PubMed 8013459