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pMPRMA42

Plasmid

#172943

Purpose

pQE30 encoding Wzz mutant i290

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i290 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA52

Plasmid

#172938

Purpose

pQE30 encoding Wzz mutant i138

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i138 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA41

Plasmid

#172935

Purpose

pQE30 encoding Wzz mutant i231

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i231 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA56

Plasmid

#172951

Purpose

pQE30 encoding Wzz mutant i66

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i66 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA46

Plasmid

#172946

Purpose

pQE30 encoding Wzz mutant i279

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i279 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA51

Plasmid

#172949

Purpose

pQE30 encoding Wzz mutant i247

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i247 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA40

Plasmid

#172942

Purpose

pQE30 encoding Wzz mutant i163

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i163 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA39

Plasmid

#172940

Purpose

pQE30 encoding Wzz mutant i128

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i128 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA44

Plasmid

#172945

Purpose

pQE30 encoding Wzz mutant i161

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i161 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA53

Plasmid

#172939

Purpose

pQE30 encoding Wzz mutant i80

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i80 mutant gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9 (BB)-2A-GFP gamma-tubulin sg

Plasmid

#104437

Purpose

Knockdown the expression of gamma-tubulin in human cells

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

gamma-tubulin sgRNA

Use

Tags

The gamma-tubulin sgRNA is coexpressed with 3xFLA…

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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IDP254

Plasmid

#87004

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

postCLAP

Use

Synthetic Biology

Tags

spGFP (1-10)

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
IDP253

Plasmid

#87003

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

preCLAP

Use

Synthetic Biology

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
IDP379

Plasmid

#87006

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

postLib-mRFP-4xboxB

Use

Synthetic Biology

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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IDP378

Plasmid

#87005

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

preLib-bfp-4xboxB

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP4K

Plasmid

#104506

Purpose

Used to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SpGAL1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pILGFP4O

Plasmid

#104510

Purpose

Used to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SpdGAL2 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGD335

Plasmid

#219499

Purpose

pAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

Insert

Eco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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HDM-Hgpm2

Plasmid

#204152

Purpose

Lentivirus helper plasmid coding for Gag/Pol

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

HIV-1 gag/pol

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HDM-tat1b

Plasmid

#204154

Purpose

Lentivirus helper plasmid coding for Tat

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

tat

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge