pMPRMA41
(Plasmid
#172935)
-
PurposepQE30 encoding Wzz mutant i231
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 172935 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepQE30
- Backbone size w/o insert (bp) 3400
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameShigella flexneri wzz i231 mutant gene
-
SpeciesShigella flexneri
-
Insert Size (bp)1162
-
Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMPRMA41 was a gift from Renato Morona (Addgene plasmid # 172935 ; http://n2t.net/addgene:172935 ; RRID:Addgene_172935) -
For your References section:
Mutagenesis and chemical cross-linking suggest that Wzz dimer stability and oligomerization affect lipopolysaccharide O-antigen modal chain length control. Papadopoulos M, Morona R. J Bacteriol. 2010 Jul;192(13):3385-93. doi: 10.1128/JB.01134-09. Epub 2010 May 7. 10.1128/JB.01134-09 PubMed 20453100