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pCDNA3 CMV sTurboID (C)-Arrestin-GFP

Plasmid

#153010

Purpose

Expresses split-TurboID C-terminal fragment fused to arrestin

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID (C)(G74)-Arrestin

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M

Plasmid

#102591

Purpose

Lentivector to express STING V155M and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

V155M

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING-342STOP

Plasmid

#102588

Purpose

Lentivector to express STING delta 342 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

delta 342

Promoter

SFFV

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

Plasmid

#126580

Purpose

A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING 368STOP

Plasmid

#102590

Purpose

Lentivector to express STING delta 368 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

delta 368

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1-Blasticidin-CAG-Flpe-ERT2

Plasmid

#68461

Purpose

AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

mammalian-codon-optimized Flpe recombinase fused with ERT2

Use

Synthetic Biology

Tags

ERT2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING-354STOP

Plasmid

#102589

Purpose

Lentivector to express STING delta 354 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

delta 354

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET empty polycistronic destination vector (2D)

Plasmid

#29774

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)

Plasmid

#198417

Purpose

Expresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeast

Depositor

Takumi Kamura

Article

Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

Insert

OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1 promoter

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM255 - sgRNA mosTI Pmlc-2_GFP

Plasmid

#159835

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI Pmlc-2_GFP

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEf1a OsTIR ires NEO pA T2BH

Plasmid

#127910

Purpose

Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

Depositor

Mark Groudine

Article

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

OsTIR

Use

Transposon

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_ST-SBP-EGFP

Plasmid

#65262

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-Golden-Gate-Esp3I-Destination

Plasmid

#51278

Purpose

CAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 WT

Plasmid

#182046

Purpose

expression of Sox2

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET empty polycistronic destination vector (2E)

Plasmid

#29775

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin

Plasmid

#199585

Purpose

Express tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vector

Depositor

Michael Lin

Article

Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

Inserts

ERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
AkaLuc
Blasticidin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-STING HAQ+V155M

Plasmid

#102601

Purpose

Express STING V55M HAQ for IFN-beta Luciferase reporter assay.

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

V155M R71H-G230A-R293Q

Promoter

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

Plasmid

#133339

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcmv3-STING-C64S,C65S-FLAG

Plasmid

#198186

Purpose

Mammalian expression of FLAG-tagged mouse STING1 C64S, C65S

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

TMEM173 (Sting1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

The cysteine in STING at position 64/65 mutates t…

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLOC CMV-IRES-Dsred-blasticidin

Plasmid

#129428

Purpose

expression of Dsred-blasticidin under CMVpromoter -IRES

Depositor

Oskar Laur

Insert

Dsred-blasticidin

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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