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Plasmid#108656PurposeA JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-JNK-EV
UseTagsXFPExpressionMammalianMutationPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6015nes
Plasmid#108657PurposeA S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-S6K-EV
UseTagsXFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6017nes
Plasmid#108655PurposeA PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-PKA-EV
UseTagsXFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-Btl2
Plasmid#117664PurposeStudy secretion efficiency of Btl2DepositorInsertpre-pro-alphaf-Btl2
UseTagsExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-E2-Crimson
Plasmid#117658PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-pro-alphaf-Btl2
Plasmid#117665PurposeStudy secretion efficiency of Btl2DepositorInsertpre-Ost1-pro-alphaf-Btl2
UseTagsExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf(I)-E2-Crimson
Plasmid#117660PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf(I)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson
Plasmid#117662PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpOst1-pro-af(MUT1)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson
Plasmid#117663PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpOst1-pro-af(MUT2)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-E2-Crimson
Plasmid#117661PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-Ost1-alphaf(I)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-Btl2
Plasmid#117667PurposeStudy secretion efficiency of Btl2DepositorInsertpre-Ost1-alphaf(I)-Btl2
UseTagsExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr-6080NES(Epac-sensor)
Plasmid#162487PurposehyBRET-Epac, an Epac biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertEpac-sensor (RAPGEF3 Human, Synthetic)
UseTagsYPet and Turquoise2GLExpressionMammalianMutationPromoterCAGAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-SEAP
Plasmid#231212PurposeSecreted Embryonic Alkaline PhosphataseDepositorInsertSEAP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BLZinCh-1
Plasmid#85082Purposemammalian vector for the expression of a dual read-out BRET-FRET sensor for intracellular Zn2+ imagingDepositorInsertBL-ZinCh-1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP N1 Ub-CRT
Plasmid#69619PurposeExpress cytoplasmic mature CRT.DepositorInsertUbiquitina-Calreticulin (mature)
UseTagsEGFP and UbiquitinExpressionMammalianMutationDeletion of signal peptide of calreticulin at the…PromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRTC2-puro
Plasmid#190567PurposeExpresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.DepositorInsertL1 (L1.3) with reporter cassette
UseTagsExpressionMammalianMutationPromoterSV40Available sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
GW1-mScarlet-Epac-GeNL
Plasmid#207874PurposeMammalian expression of the FRET/BRET cAMP sensorDepositorInsertmScarlet-hEpac1(149-881)-mNeonGreen-NanoLuc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CA-0140 CMVTO-Renin-ECL-CD4-tevs-RXR6.2
Plasmid#231199PurposeER-retained reninDepositorInsertRenin-ECL-CD4-tevs-RXR6.2 (REN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRARα.hRXRα
Plasmid#135411PurposeHuman Retinoic Acid Receptor-alpha and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vectorDepositorInsertsHuman retinoic acid receptor-alpha
Human retinoid X receptor-alpha
UseTagsExpressionMammalianMutationPromoterCMV and SV40Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRARγ.hRXRα
Plasmid#135416PurposeHuman Retinoic Acid Receptor-gamma and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vectorDepositorInsertsHuman retinoic acid receptor-gamma
Human retinoid X receptor-alpha
UseTagsExpressionMammalianMutationPromoterCMV and SV40Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRARβ.hRXRα
Plasmid#135415PurposeHuman Retinoic Acid Receptor-beta and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vectorDepositorInsertsHuman retinoic acid receptor-beta
Human retinoid X receptor-alpha
UseTagsExpressionMammalianMutationPromoterCMV and SV40Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGK112
Plasmid#228127PurposeExpress node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cellsDepositorInsertRetron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK111
Plasmid#228126PurposeExpress node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) EMX1 gene in human cellsDepositorInsertRetron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (SNP + PAM recode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-6013x
Plasmid#108654PurposeAn HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertRaichu-EV
UseLentiviralTagsXFPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-6085x
Plasmid#108653PurposeA tyrosine kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertPicchuEV
UseLentiviralTagsXFPExpressionMammalianMutationPromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
CA-0192 CMVTO-Renin(mouse)-ECL-CD4-tevs-RXR6.2
Plasmid#231204PurposeER-retained mouse reninDepositorInsertRenin(mouse)-ECL-CD4-tevs-RXR6.2 (Ren1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0193 CMVTO-Renin(H2M)-ECL-CD4-tevs-RXR6.2
Plasmid#231203PurposeER-retained H2M reninDepositorInsertRenin(H2M)-ECL-CD4-tevs-RXR6.2 (REN Human)
UseTagsExpressionMammalianMutationL147I, R148H, I203V, L290VPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAGK58
Plasmid#228118PurposeExpress node 814 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 814, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAGK65
Plasmid#228123PurposeExpress node 1795 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 1550, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK59
Plasmid#228119PurposeExpress node 1429 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 1429, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK57
Plasmid#228117PurposeExpress node 482 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 482, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK56
Plasmid#228116PurposeExpress node 412 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 412, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK61
Plasmid#228121PurposeExpress node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK60
Plasmid#228120PurposeExpress node 1438 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 1438, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK62
Plasmid#228122PurposeExpress node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK55
Plasmid#228114PurposeExpress node 2 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 2, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
gp64-mVEGF-C-HisTag-pENTR
Plasmid#64065PurposePlasmid encodes mature form of murine VEGF-C preceded by baculoviral gp64 secretion signal; entry vector to Gateway cloning system.DepositorInsertvascular endothelial growth factor C (Vegfc Mouse)
UseTagsHis-tagExpressionInsectMutationPromoterAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
HBM-mVEGF-C-HisTag-pENTR
Plasmid#64066PurposePlasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.DepositorInsertvascular endothelial growth factor C (Vegfc Mouse)
UseTagsHis-tagExpressionInsectMutationPromoterAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA
Plasmid#164471PurposeExpresses sgRNA targeting human GJA1 locusDepositorInsertGap junction alpha-1 (GJA1) (GJA1 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Str-Golgin84_VSVG-SBP-EGFP
Plasmid#65305Purposesynchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)DepositorInsertGolgin-84 (GOLGA5 Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only