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Plasmid#124064PurposeControl protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATLDepositorInsertcytXlATL2-R232Q
UseTagsExpressionBacterialMutationPromotertacAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-cytXlATL2
Plasmid#124063PurposeFragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.DepositorInsertcytXIATL2
UseTagsExpressionBacterialMutationPromotertacAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP
Plasmid#124066PurposeE. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.DepositorInsertcytATL-R232Q-sfGFP
UseTagssfGFPExpressionBacterialMutationR232QPromotertrcAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pProEx-Htb-cytATL2-6Gly-sfGFP
Plasmid#124065PurposeE. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.DepositorInsertcytATL-sfGFP
UseTagssfGFPExpressionBacterialMutationPromotertrcAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-peripherin-CTS
Plasmid#86070PurposeA bacterial expression plasmid encoding peripherin ciliary targeting signal with N-terminus GST-tag.DepositorInsertPeripherin (prph2.S Frog)
UseTagsGSTExpressionBacterialMutationcontains the region from 317-336 of peripherinPromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
x.Amylase
Plasmid#87132PurposeTo make in situ hybridization probe for Xenopus amylase.DepositorInsertAmylase
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSI HA Nup53xl
Plasmid#86000Purposemammalian expression of HA tagged Xenopus laevis Nup53DepositorInsertXenopus laevis Nup53 (nup35.L Frog)
UseTagsHAExpressionMammalianMutationcodon optimzed for bacterial expressionPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSI myc Nup53xl
Plasmid#86001Purposemammalian expression of myc tagged Xenopus laevis Nup53DepositorInsertXenopus laevis Nup53 (nup35.L Frog)
UseTagsmycExpressionMammalianMutationcodon optimzed for bacterial expressionPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
x.hdac8-ORF
Plasmid#87134Purposemammalian expression of hdac8DepositorInserthdac8 (hdac8.L Frog)
UseTagsExpressionMammalianMutationPromotersimian CMV IE94Available sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
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Xlim-3 (lhx3)
Plasmid#74139PurposePartial cDNA clone for Xlim-3 (lhx3), named LH23-1. Cloned in BS KS+. To prepare RNA, sense: HindIII/T7; antisense: BamHI/T3.DepositorInsertlhx3 (lhx3 Frog)
UseTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Xlim-1 (Lhx1) 3m
Plasmid#74136PurposeThis is a mutant of Xlim-1/Lhx1 with one point mutation in each of the two LIM domains. Cloned in XbetaM. To prepare sense RNA: SalI/SP6.DepositorInsertlhx1 3m
UseExpression in frog embryoTagsExpressionMutationPromoterAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Xp75 CDD
Plasmid#61283PurposeExpresses Xenopus p75 CDD domainDepositorInsertp75 CDD (p75NTRa Frog)
UseXenopus expressionTagsExpressionMammalianMutationlacks all but the first five amino acids of the p…PromoterCMVAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Xl-VSP1 R152Q
Plasmid#51881Purposeexpression of Xl-VSP1 mutant in Xenopus oocytes or mammalian cellsDepositorInsertVSP1 R152Q (tpte2 Frog)
UseXenopus oocyte expressionTagsExpressionMammalianMutationR152Q: near the extracellular side of the fourth …PromoterCMVAvailable sinceMay 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Xl-VSP2 GFP
Plasmid#51886Purposeexpression of Xl-VSP2-GFP fusion in Xenopus oocytes or mammalian cellsDepositorInsertVSP2
UseXenopus oocyte expressionTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
XE174 Xenopus Wnt-5A-myc-pCDbeta-G
Plasmid#16873DepositorInsertWnt-5A (wnt5a.S Frog)
UseXenopus expressionTagsmycExpressionMutationPromoterAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
XE5 Xwnt-5A Hind RNA
Plasmid#16872DepositorInsertXwnt-5A Hind RNA (wnt5a.S Frog)
UseXenopus expressionTagsExpressionMutationStarts with AA 14 (see comments)PromoterAvailable sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
M61 pPET28c-Xenopus beta catenin domains R10C
Plasmid#17202DepositorInsertbeta catenin (ctnnb1.L Frog)
UseTagsHISExpressionBacterialMutationdomains R10CPromoterAvailable sinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
M60 pPET28a-Xenopus beta catenin domains NR7
Plasmid#17201DepositorInsertbeta catenin (ctnnb1.L Frog)
UseTagsHISExpressionBacterialMutationdomains NR7PromoterAvailable sinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -