x.Setd7
(Plasmid
#87133)
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Purposemammalian expression of Setd7
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87133 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2++
- Backbone size w/o insert (bp) 4113
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSetd7
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SpeciesX. laevis (frog)
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Insert Size (bp)1157
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Entrez Genesetd7.L (a.k.a. XELAEV_18005560mg)
- Promoter simian CMV IE94
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
x.Setd7 was a gift from Francesca Spagnoli (Addgene plasmid # 87133 ; http://n2t.net/addgene:87133 ; RRID:Addgene_87133) -
For your References section:
The histone methyltransferase Setd7 promotes pancreatic progenitor identity. Kofent J, Zhang J, Spagnoli FM. Development. 2016 Oct 1;143(19):3573-3581. Epub 2016 Aug 30. 10.1242/dev.136226 PubMed 27578787