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pLNC Wnt-3aHA

Plasmid

#18030

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3a (Wnt3a Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only

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pIVT-myc-T58A-S62D

Plasmid

#138540

Purpose

For making myc-T58A-S62D RNA

Depositor

Carmen Williams

Insert

Myelocytomatosis oncogene T58A S62D (Myc Mouse)

Use

In vitro transcription

Tags

Expression

Mutation

Threonine 58 changed to alanine and serine 62 cha…

Promoter

T7

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe Nf2 wild-type

Plasmid

#14116

Depositor

Tyler Jacks

Article

Johnson et al Oncogene. 2002 Sep 5. 21(39):5990-7.

Insert

Nf2 (Nf2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pBa-FRB-3myc-KIF1A tail 391-1698

Plasmid

#64286

Purpose

KIF1A aa 391-1698 with N-terminal FRB and triple myc tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

KIF1A (Kif1a, Kif1a Rat, Mouse)

Use

Tags

3myc and FRB

Expression

Mammalian

Mutation

aa 391-1698, 5'end mostly matches rat, 3…

Promoter

chicken Beta Actin

Available since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-DYRK3-2A-mCherry

Plasmid

#118287

Purpose

Expresses mouse DYRK3 tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.

Depositor

Vance Lemmon

Article

Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

Insert

dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (Dyrk3 Mouse)

Use

AAV

Tags

2A peptide and mCherry

Expression

Mammalian

Mutation

Promoter

CMV with beta global intron

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-mCep290

Plasmid

#27381

Depositor

Joseph Gleeson

Article

Valente et al Nat Genet. 2006 Jun . 38(6):623-5.

Insert

Cep290 (Cep290 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

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mRIP3 GFP RHIM mut

Plasmid

#41384

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (Ripk3 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

RHIM mutant

Promoter

CMV

Available since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pET21-Drp1

Plasmid

#72927

Purpose

Bacterial expression of mouse Drp1 isoform b (699amino acids) with C-terminal His-tag and PreScission protease cleavage site

Depositor

David Chan

Article

Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

Insert

Drp1 (Dnm1l Mouse)

Use

Tags

6xHis-tag and PreScission Protease site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTET2 (CD)

Plasmid

#89736

Purpose

Expresses the CD-domain truncation to mouse TET2 in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

TET2 (Tet2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

CD (cysteine-rich, dioxygenase domain) truncation…

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV14-mULK1

Plasmid

#24301

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
Anti-GluN2A [N327/95R]

Plasmid

#177531

Purpose

Mammalian Expression Plasmid of anti-GluN2A/NR2A glutamate receptor (Rat). Derived from hybridoma N327/95.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GluN2A/NR2A glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Grin2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV4Neo-murine IL-17RC(FL)

Plasmid

#46864

Purpose

expresses mouse IL-17RC (all exons)

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RC (Il17rc Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA flag-Pcbp2

Plasmid

#184028

Purpose

Codon optimized Pcbp2 clone with N-terminal flag tag

Depositor

Peter Stoilov

Article

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Pcbp2 (Pcbp2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Codon optimized

Promoter

CMV

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRFP703-Myosin

Plasmid

#79995

Purpose

LC-Myosin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

LC-Myosin (Myl9 Mouse)

Use

Tags

miRFP703

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-mMCL1-WT

Plasmid

#45817

Depositor

Joseph Opferman

Article

Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

Insert

mMCL-1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-HA-mSTING (V154M)

Plasmid

#214159

Purpose

Transient expression of gain-of-function STING mutant

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (V154M) (Sting1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

N153S

Promoter

CMV

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-mSTING (N153S)

Plasmid

#214158

Purpose

Transient expression of gain-of-function STING mutant

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (N153S) (Sting1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

N153S

Promoter

CMV

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA-Cre-GpNLuc-sgmSTING

Plasmid

#208386

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine STING gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgSTING (Sting1 Mouse)

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Sema3c(L)-AP-His

Plasmid

#72015

Purpose

Expresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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CAG-NeuroD6iresGFP

Plasmid

#45027

Depositor

Connie Cepko

Article

Cherry et al J Neurosci. 2011 May 18;31(20):7365-79.

Insert

NeuroD6 (Neurod6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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