pCMV4Neo-murine IL-17RC(FL)
(Plasmid
#46864)
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Purposeexpresses mouse IL-17RC (all exons)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46864 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV4-Neo
- Backbone size w/o insert (bp) 5750
- Total vector size (bp) 8000
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIL-17RC
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SpeciesM. musculus (mouse)
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Insert Size (bp)2200
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Entrez GeneIl17rc (a.k.a. 1110025H02Rik, Gm19850, IL-17RC, IL17-RC, IL17-RL, Il17rl)
- Promoter CMV
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Tag
/ Fusion Protein
- Myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer GAACCGTCAGAATTG
- 3′ sequencing primer CTTCCAAGGCCAGGAGAG (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV4Neo-murine IL-17RC(FL) was a gift from Sarah Gaffen (Addgene plasmid # 46864 ; http://n2t.net/addgene:46864 ; RRID:Addgene_46864) -
For your References section:
Distinct functional motifs within the IL-17 receptor regulate signal transduction and target gene expression. Maitra A, Shen F, Hanel W, Mossman K, Tocker J, Swart D, Gaffen SL. Proc Natl Acad Sci U S A. 2007 May 1;104(18):7506-11. Epub 2007 Apr 24. 10.1073/pnas.0611589104 PubMed 17456598