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Anti-SUR2B [N323B/20R]

Plasmid

#114480

Purpose

Mammalian Expression Plasmid of anti-SUR2B (Rat). Derived from hybridoma N323B/20.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SUR2B (Rattus norvegicus) recombinant mouse monoclonal antibody (Abcc9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pNJP

Plasmid

#115494

Purpose

Expression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

Insert

NLS-iRFP-NLS-P2A-JNK KTR-mCherry-P2A-mKO-MK2 (MAPK14, MAPK8 Synthetic, Mouse, Human)

Use

Tags

iRFP, mCherry, and mKO

Expression

Mammalian

Mutation

mCherry S227F, mCherry G229R, mKO V211G

Promoter

CAG

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-miR9/9*/124

Plasmid

#177807

Purpose

The pre-miR-9/9* and pre-miR-124 sequences have been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-9/9*/124

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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GluA3 (px330:2-guideCas9)

Plasmid

#169424

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluA3 TKIT donor

Plasmid

#169425

Purpose

Donor DNA for TKIT knockin of SEP to GluA3 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (W131G)

Plasmid

#169724

Purpose

Mammalian Expression of mouse SPOP W131G

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

W131G

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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ATF4 6: mCD36.GFP

Plasmid

#21853

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

mCD36 (Cd36 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Only the first 13 AA of CD36 are fused to GFP

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pET28_mouse-Caspase-11_enzymatic-domain

Plasmid

#183382

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-11 (AA100-373) (Casp4 Mouse)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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Cavin4-mCherry

Plasmid

#176007

Purpose

Mammalian expression of Mouse Cavin4-mCherry. Parton lab clone JSG

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4 (Cavin4 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pMX-SHP1C-ires-hCD8

Plasmid

#183720

Purpose

Expresses the myc-tagged catalytic domains of wild-type SHP-1.

Depositor

Takeshi Tsubata

Article

Alborzian Deh Sheikh et al J Immunol. 2021 Jun 1;206(11):2544-2551.

Insert

SHP-1

Use

Retroviral

Tags

Expression

Mutation

catalitic domain alone

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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Let-7b-GFP

Plasmid

#25404

Depositor

Sean Morrison

Article

Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

Insert

Mirlet7b (Mirlet7b Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
ACTIN-SOX2-PGK Cre

Plasmid

#59019

Purpose

Bicistronic Lentivirus expressing sox2 and Cre

Depositor

Trudy Oliver

Article

Mukhopadhyay et al Cell Rep. 2014 Jul 10;8(1):40-49. doi: 1

Insert

Sox2 (Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

B-actin

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pLX304_zeo_mmFbxw7_R505C

Plasmid

#160104

Purpose

Expresses murine Fbxw7 alpha with the R505C loss-of-function mutation in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7 alpha R505C (Fbxw7 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

R505C

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Ezr-eGFP

Plasmid

#176856

Purpose

Expresses Ezrin fused with eGFP at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Ezr-GFP

Use

AAV

Tags

GFP

Expression

Bacterial

Mutation

Promoter

gfaABC1D

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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AAV2-miniSOG-VAMP2-T2A-mCherry

Plasmid

#50970

Purpose

AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-T2A-mCherry) construct

Depositor

Roger Tsien

Article

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Insert

miniSOG-VAMP2-T2A-mCherry (Vamp2 Arabdopsis thaliana, Mouse)

Use

AAV

Tags

T2A-mCherry and miniSOG

Expression

Mutation

Promoter

human synapsin promoter

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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SF-1

Plasmid

#28180

Depositor

Bernard Schimmer

Article

Frigeri et al Endocrinology. 2002 Oct . 143(10):4031-7

Insert

SF-1 (Nr5a1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag Myb

Plasmid

#66980

Purpose

Expression plasmid for mouse Myb; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Myb (Myb Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 15, 2015

Availability

Academic Institutions and Nonprofits only
RCAS-K-ras

Plasmid

#11549

Depositor

Sandra Orsulic

Article

Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

Insert

v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (Kras Mouse)

Use

Retroviral

Tags

Expression

Mutation

G12D

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pBABE-hygro-2xMyc-Rac1-WT

Plasmid

#128580

Purpose

For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn LAMP1-msGFP (JV012)

Plasmid

#179728

Purpose

Lentiviral expression of LAMP1-msGFP for use as a lysosomal marker

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

LAMP1 (Lamp1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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