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pMR-A-5

Plasmid

#138519

Purpose

P3c

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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myc-hIFITM3-P17A,P18A

Plasmid

#104364

Purpose

Expresses human IFITM3-P17A,P18A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al PLoS Pathog. 2015 Aug 11;11(8):e1005095.

Insert

IFITM3 (IFITM3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

mutated amino acids as follows: P17A,P18A

Promoter

CMV

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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Myc-CICf_AEA

Plasmid

#48188

Purpose

Expresses AEA mutated Cic

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Capicua (Cic Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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ABE8e

Plasmid

#138489

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-3XKD/Y1699C

Plasmid

#25367

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Tags

2XMyc

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
Equalizer-L plasmid

Plasmid

#169732

Purpose

Equalizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pCMV-tetO2

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEF-ABEmax

Plasmid

#164415

Purpose

EF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

ABEmax

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pC0079 RESCUE-S

Plasmid

#130662

Purpose

pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

dRanCas13b-ADAR2dd

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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enAsABE8e

Plasmid

#138506

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-dAsCas12a (En)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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m6A-Tracer-NES

Plasmid

#159607

Purpose

Constitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

m6A-Tracer-NES

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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ND4.2-Left TALE-G1397-N-DddA11-mCherry

Plasmid

#179682

Purpose

Expression of base editor in mammalian cells and for cell sorting

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

S1330I A1341V N1342S E1370K T1380I

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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Tag5Amyc-GSK3b WT

Plasmid

#16260

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

GSK3 beta (GSK3B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-MKL1

Plasmid

#11978

Purpose

For mammalian expression of human MKL1 with a 3x FLAG tag

Depositor

Ron Prywes

Article

Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

Insert

MKL1 (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

N579S T716A

Promoter

CMV

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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TPI-PTC160

Plasmid

#130698

Purpose

Expresses TPI-PTC160 NMD reporter (It has a Premature termination codon (PTC) at 160th Amino acid)

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

TPI-PTC160 (TPI1 Human)

Use

Tags

Expression

Mammalian

Mutation

PTC160

Promoter

Pcmv

Available since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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xCas9 3.7

Plasmid

#108379

Purpose

Mammalian expression vector for xCas9 3.7

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9 3.7

Use

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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SaABE8e

Plasmid

#138500

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSaCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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nLbCas12a

Plasmid

#213745

Purpose

For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

nLbCas12a

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R, R1138A

Promoter

CMV

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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LbCas12a-D156R

Plasmid

#213744

Purpose

For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

LbCas12a

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R

Promoter

CMV

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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Beta-Globin-WT

Plasmid

#130700

Purpose

Expresses Beta-Globin WT NMD reporter

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

Beta Globin (HBB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Pcmv

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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SpCas9 nickase for Split-PE (pJUL2497)

Plasmid

#190104

Purpose

Expresses nickase SpCas9(H840A) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

nSpCas9

Use

Tags

XTEN-bpNLS and bpNLS

Expression

Mammalian

Mutation

H840A

Promoter

CMV

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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