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Plasmid#91777PurposeMammalian expression of low affinity calcium-sensing fluorescent proteinDepositorInsertGCaMP6-150
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V
Plasmid#119723PurposeAAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorInsertsCaMPARI2
CaMPARI2
UseAAVTagsNES-hisExpressionMammalianMutationF391W, L398VPromoterhSyn1Available sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
NOTCH3-bio-His
Plasmid#53343PurposeExpresses full-length Neurogenic locus notch homolog protein 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertNOTCH3 (NOTCH3 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
hZMPSTE24-3FLAG_pQCXIP
Plasmid#89759PurposeHuman ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.DepositorInsertHomo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
UseRetroviral; Bicistronic expression: puromycin re…Tags9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…ExpressionMammalianMutationY253H in ZMPSTE24 (see depositor comments below)PromoterCMVAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgACSL4
Plasmid#186024Purposeknock out ACSL4 in mammalian cellsDepositorInsertAcyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-DIO-TRPV1-P2A-mCherry
Plasmid#200831PurposeIn the presence of Cre, drives neuron-specific expression of TRPV1 with fluorescence reporter mCherryDepositorInsertTRPV1-P2A-mCherry (Trpv1 Rat)
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-DIO-BiPOLES-mCerulean
Plasmid#154949PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVTagsExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6-210
Plasmid#91778PurposeMammalian expression of low affinity calcium-sensing fluorescent proteinDepositorInsertGCaMP6-210
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-NpHR3.3-EYFP
Plasmid#137151PurposeViral expression of NpHR3.3-EYFPDepositorHas ServiceAAV8InsertNpHR3.3-EYFP
UseAAVTagsExpressionMutationW179FPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-E2F1
Plasmid#37141DepositorInsertE2F1 (E2f1 Fly)
UseGatewayTagsExpressionMutationPromoterAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAVTRE3_Clover
Plasmid#135179PurposeAAV expression of TRE(TRE3G)-driven, Clover expression for fluorescent lablingDepositorInsertsClover
TRE3G
UseAAVTagsExpressionMammalianMutationa variant of GFPPromoterTRE3GAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF3018
Plasmid#144494PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNEUROG3 (NEUROG3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX307 DEDD
Plasmid#117744PurposeOpen reading frame vector encoding DEDDDepositorInsertDEDD1 (DEDD Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV superYFP-AURKA-mTurq2
Plasmid#157772PurposeExpression of AuroraA kinase biosensor under CMV promoterDepositorInsertAURKA (AURKA Human)
UseTagsmTurquoise2 and superYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
hSyn-somAion-citrine
Plasmid#192570PurposeAAV-vector for optogenetic long-term silencing of neuronsDepositorInsertsomAion
UseAAVTagsCitrine - soma-targeting motif from Kv2.1 channelExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…Promoterhuman synapsinAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Human b4 nicotinic receptor subunit EM construct
Plasmid#163961PurposepEZT-BM vector with beta4 gene after CMV promoter, published EM constructDepositorInsertHuman beta4 nicotinic acetylcholine receptor subunit EM construct
UseBaculovirus (bacmam) production and mammalian exp…TagsStrepII tag and apocytochrome b(562)RIL (BRIL)ExpressionMammalianMutationPro341-Ser395 replaced with apocytochrome b(562)R…PromoterCMVAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF3023
Plasmid#144499PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNEUROD6 (NEUROD6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-mCherry-LacR-BRCA1 1366-1437
Plasmid#71118Purposemammalian expession of mCherry-LacR-BRCA1 1366-1437DepositorInsertBRCA1 (BRCA1 Human)
UseTagsmCherryExpressionMammalianMutationcontains amino acids 1366-1437 of BRCA1PromoterCMV/TOAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-PTRE-tight-flex-hM3Dq-mCherry-WPRE-pA
Plasmid#115161PurposeThis plasmid is for use with neuronal cell-type selective activity tagging. The hM3Dq receptor expression requires both neural activity and Cre recombinase.DepositorInsertsPTRE - tet activator responsive promoter
flex sequence
hM3dq-mCherry (inverted)
flex sequence
UseAAVTagshM3Dq-mCherryExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40
Plasmid#101062PurposeAAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorInsertCaMPARI2-H396K
UseAAVTagsFLAG-HA-myc and NES_hisExpressionMutationPromoterhsyn (synapsin-1)Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) NRP1 b1 (273-427)
Plasmid#162734PurposeBacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.DepositorInsertHuman NRP1 b1 domain residues 273-427
UseTagsExpressionBacterialMutationCodon optimisedPromoterAvailable sinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a(+) NRP1 b1b2 (273-586)
Plasmid#162733PurposeBacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.DepositorInsertHuman NRP1 b1b2 domains (residues 273-586)
UseTags6HisExpressionBacterialMutationCodon optimisedPromoterAvailable sinceMarch 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV.CMV.SV40.THBS1-deltaCTD-HA.SV40(polyA)
Plasmid#156163PurposeExpresses truncated (AA 1-954) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminusDepositorInsertThrombospondin-1 (Thbs1 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationTruncated THBS1 (amino acid residues 1-954)PromoterCMVAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hZBTB7B
Plasmid#180380Purposelentiviral transduction of human ZBTB7B geneDepositorInsertZBTB7B (ZBTB7B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO Myc ArB WT RNAiR
Plasmid#59806PurposeAllows the integration of Myc ArB in the genome and Tet-inducible expression.DepositorInsertAurora B (AURKB Human)
UseTagsMycExpressionMammalianMutationRNAi resistantPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(-)-GPI
Plasmid#184335PurposeExpression of GPI-anchored form of mouse Nrx3-alpha containing the alternative splice site 5 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,3,DepositorInsertNrxn3-alpha-GPI (Nrxn3 Mouse)
UseTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-T0-Flag-HROB
Plasmid#135298PurposeExpresses Flag-tagged HROB in mammalian cellsDepositorInsertHROB (HROB Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV AURKA-mTurq2
Plasmid#157767PurposeExpression of AuroraA kinase fused to mTurquoise2 under CMV promoterDepositorInsertAURKA (AURKA Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.SV40.THBS1-HA-KDEL.SV40(polyA)
Plasmid#156164PurposeExpresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus and KDEL (ER retention) sequenceDepositorInsertThrombospondin-1 (Thbs1 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationTHBS1 with addition of ER-retention sequence KDELPromoterCMVAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S87E
Plasmid#36059DepositorInsertalpha-synuclein S87E (SNCA Human)
UseTagsExpressionBacterialMutationS87EPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-Egr3-EYFP-WPRE
Plasmid#186417PurposeOverexpression of Egr3 transcript variant 1 CDS under EF-1α promoterDepositorInsertEarly growth response 3 (Egr3 Mouse)
UseAAVTagsEYFPExpressionMutationPromoterEF-1-alphaAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S87A
Plasmid#36058DepositorInsertalpha-synuclein S87A (SNCA Human)
UseTagsExpressionBacterialMutationS87APromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/T0-GFP-BRCA1 1364-1437
Plasmid#71119PurposeMammalian expression of GFP-BRCA1 1364-1437DepositorInsertBRCA1 (BRCA1 Human)
UseTagsGFPExpressionMammalianMutationcontains amino acids 1364-1437 of BRCA1PromoterCMV/TOAvailable sinceDec. 9, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Kv7.4 (1-645, Δ368-523)/HA tag/pcDNA3.1
Plasmid#111459PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHa TagExpressionMammalianMutationKv7.4 (1-645, Δ368-523), C643APromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCh2007_pLenti_Syn_mChe-cdc42E7
Plasmid#118620Purposeexpression of mChe-tagged cdc42E7 under pSyn promoter for primary neuronsDepositorInsertcdc42E7 (Cdc42 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationchanged cagtctg to ggcataa (667 - 673 nt in 3…Promotersynapsin I (rat)Available sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX62
Plasmid#46854PurposeTOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCEDepositorInsertattB-Puro-2A and Hupki p53 G245S (TP53 Human, Mouse)
UseTagsExpressionMammalianMutationG245SPromoterAvailable sinceAug. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTBL681 CHYRON4 integration construct
Plasmid#126449PurposeTo integrate the CHYRON4 locus at AAVS1 in human cells.DepositorInsertspU6/3xLacO-CHYRON4 hgRNA
pCMV-puro
UseTagsExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6/3xLacOAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-cGAS R71/75E C396/7A-HA
Plasmid#130920PurposeExpresses human cGAS with R71/75E and C396/397A point mutations; Puromycin selection markerDepositorInserthuman cGAS R71/75E C396/7A
UseTagsHAExpressionMammalianMutationaa 71 and 75 mutated to glutamate; aa 396 and 397…PromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 TO FRT Myc ArB K106R
Plasmid#59807PurposeAllows the integration of Myc ArB K106R in the genome and Tet-inducible expressionDepositorInsertAurora B (AURKB Human)
UseTagsMycExpressionMammalianMutationK106R, kinase mutantPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-T0-eGFP-HROB
Plasmid#135299PurposeExpresses eGFP-tagged HROB in mammalian cellsDepositorInsertHROB (HROB Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTBL649 CHYRON2 integration construct
Plasmid#126446PurposeTo integrate the CHYRON2 locus at AAVS1 in human cells.DepositorInsertspU6-CHYRON2 hgRNA
pCMV-puro
UseTagsExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AB (325-557, Δ 368-523)/pET28HMT
Plasmid#111052PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AB (325-557, Δ 368-523)PromoterT7Available sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
PANK2 gRNA (BRDN0001149459)
Plasmid#76809Purpose3rd generation lentiviral gRNA plasmid targeting human PANK2DepositorInsertPANK2 (PANK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SLC30A10-delta105-107
Plasmid#65205PurposeConstitutive expression of SLC30A10 delta 105-107 in mammalian cellsDepositorInsertSLC30A10 (SLC30A10 Human)
UseTagsFlagExpressionMammalianMutationdelta 105-107 amino acidsPromoterCMVAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-E2F1-PIP3A
Plasmid#37142DepositorInsertE2F1-P!P3A (E2f1 Fly)
UseGatewayTagsExpressionMutationI153, Y156, Y157 to AlaninePromoterAvailable sinceJuly 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV.E2.ChETA
Plasmid#220310PurposeDirects ChETA optogenetic actuator to fast spiking interneurons in cortex. Allows for single APs to be evoked.DepositorInsertChETA
UseAAVTagsExpressionMammalianMutationPromoterS5E2Available sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE
Plasmid#141236PurposeAAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)DepositorInsertNES-jRGECO1a
UseAAV and Cre/Lox; Cre-offTags6xHIS tag and nuclear export signalExpressionMutationPromoterhuman elongation factor-1 alpha (EF-1 alpha)Available sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_CCK1.0
Plasmid#208673PurposeExpresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in neuronsDepositorInsertGPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 R57A, R59A pMT2
Plasmid#206112Purposeexpression of rat Cav2.1 calcium channel with R57A and R59A mutations that prevent G-protein modulationDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationR57A, R59APromoterAd MLP/TPL/SV40Available sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 D122A pcDNA3
Plasmid#206068Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1DepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only