Skip to main content
Addgene
Showing: 4261 - 4290 of 148416 results

  1. pCambia-loxKan

    Plasmid
    #199590
    Purpose
    basic plant vector with a plant-selectable kanamycin resistance cassette flanked by both loxP and FRT sites for sustainable plant engineering with subsequent marker removal possibility
    Depositor
    Andreas Stuermer
    Insert
    FRT-lox66-NosP-KanR-NosT-lox71-FRT
    Use
    Cre/Lox
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Nos
    Available since
    July 5, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199590-plasmid-map-sequence-id-399425

    Enlarge

  2. PEA1-Puro

    Plasmid
    #171991
    Purpose
    Delivers all prime editing nickase components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171991-plasmid-map-sequence-id-338127

    Enlarge

  7. pAAV hSyn GFP-Cre

    Plasmid
    #175381
    Purpose
    expresses GFP-Cre under the control of a human synapsin promoter
    Depositor
    Jason Aoto
    Insert
    GFP-Cre recombinase
    Use
    AAV
    Tags
    Cre is fused to GFP. GFP is fused to additional n…
    Expression
    Mutation
    Promoter
    human synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    175381-plasmid-map-sequence-id-348392

    Enlarge

  14. pEMS2115

    Plasmid
    #49140
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414
    Insert
    ssAAV-Ple155-emGFP-WPRE
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available since
    May 10, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    49140-plasmid-map-sequence-id-245687

    Enlarge

  16. c-Flag pcDNA3

    Plasmid
    #20011
    Purpose
    This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.
    Depositor
    Stephen Smale
    Article
    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20011-plasmid-map-sequence-id-372933

    Enlarge

  17. OPRK1-Tango

    Plasmid
    #66462
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    OPRK1 (OPRK1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66462-plasmid-map-sequence-id-157905

    Enlarge

  21. pGL4.22-PGK1-HRE::dLUC

    Plasmid
    #128095
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Article
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Insert
    Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Mutation
    Promoter
    inserted PGK1 hypoxia response elements (HRE) (x3)
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128095-plasmid-map-sequence-id-249667

    Enlarge

  27. pAAV-SynI-CreOn-FlpOff-Kir2.1-P2A-EGFP

    Plasmid
    #176278
    Purpose
    Viral vector for co-expression of Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.
    Depositor
    Martin Riccomagno
    Article
    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    Kir2.1-P2A-EGFP (Kcnj2 Mouse, Synthetic)
    Use
    AAV and Cre/Lox
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin I
    Available since
    Jan. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    176278-plasmid-map-sequence-id-350556

    Enlarge

Showing: 4261 - 4290 of 148416 results