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Plasmid#52974Purposeinsect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)DepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationT196A LKB1 phosphorylation defective mutantPromoterHsp70Available sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 K70M
Plasmid#52973Purposeinsect expression of SIK3 K70M (kinase dead mutant)DepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationK70M kinase dead mutantPromoterHsp70Available sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ths pUAST-HA
Plasmid#69769PurposeThisbe (FGF8-like-1) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertThisbe (FGF8like-1)
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationEncodes amino acids 1-739 of Thisbe (NP_610701.2)…Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]
Plasmid#149426PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.DepositorInsertsU6.3-gRNAs[Sxl, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10
UseCRISPRTagseGFPExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB_NSnoFbox-vhhGFP4
Plasmid#35578DepositorInsertNSnoFbox-vhhGFP4 (slmb Fly)
UseTagsExpressionInsectMutationF box domain of slmb deleted.PromoterAvailable sinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) dRhoA V14 (CT#846)
Plasmid#13949DepositorInsertRhoA (Rho1 Fly)
UseRetroviral; Avian expressionTagsExpressionMutationG14V mutationPromoterAvailable sinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pUASTLOTattB_mCherry-CD8-vhhGFP
Plasmid#163930PurposeExpression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) dRhoA N19 (CT#847)
Plasmid#14002DepositorInsertRhoA (Rho1 Fly)
UseRetroviral; Avian expressionTagsExpressionMutationT19NPromoterAvailable sinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
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LN-entry
Plasmid#128012PurposeVector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertLN-3xFlag
UseLamdan entry vectorTagsLN-3xFlagExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-PtW-eSpCas9
Plasmid#133357PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expressionDepositorInserteSpCas9
UseTagsHA-tagged eSpCas9ExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET19b-MED17
Plasmid#15412DepositorInsertMED17 (MED17 Human)
UseTagsHisExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_DUM1H01
Plasmid#71090PurposeGateway entry cloneDepositorInsertnej (nej Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGex4t2 with 244-458AA SUUR fragment
Plasmid#112989PurposeExpresses fusion of GST and amino acids 244-458 of Drosophila SUURDepositorInsertSuUR (SuUR Fly)
UseTagsExpressionBacterialMutationamino acids 244-458PromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRpr150-LUC
Plasmid#85700Purposeluciferase reporter driven by a DNA damage responsive cis-regulatory sequence from the reaper locus, containing a p53 binding site (Rpr150 enhancer)DepositorInsertRpR 150 enhancer and hsp70 minimal promoter
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterRpR 150 enhancer and hsp70 minimal promoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMM1157
Plasmid#112534PurposepZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanRDepositorInsertsHrtR
luxCDABE
UseTagsExpressionBacterialMutationPromoterPL(HrtO) and ProDAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pnEA-vH_His-TEV-DSep1
Plasmid#174493Purposebacterial expression of Drosophila Sep1DepositorInsertSep1 (Sep1 Fly)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only