pGex4t2 with 244-458AA SUUR fragment
(Plasmid
#112989)
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PurposeExpresses fusion of GST and amino acids 244-458 of Drosophila SUUR
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112989 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX 4t2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSuUR
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SpeciesD. melanogaster (fly)
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Mutationamino acids 244-458
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Entrez GeneSuUR (a.k.a. Dmel_CG7869, CG7869, Dmel\CG7869, Dromel_CG7869_FBtr0076203_suur_mORF, SU(UR), SUUR, Su(UR), Su(UR)ES, su(UR)ES)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGex4t2 with 244-458AA SUUR fragment was a gift from Terry Orr-Weaver (Addgene plasmid # 112989 ; http://n2t.net/addgene:112989 ; RRID:Addgene_112989) -
For your References section:
DNA copy-number control through inhibition of replication fork progression. Nordman JT, Kozhevnikova EN, Verrijzer CP, Pindyurin AV, Andreyeva EN, Shloma VV, Zhimulev IF, Orr-Weaver TL. Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10.1016/j.celrep.2014.10.005. Epub 2014 Oct 30. 10.1016/j.celrep.2014.10.005 PubMed 25437540
