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Plasmid#160732PurposeMini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.DepositorInsertVchCAST donor DNA (Mini-Tn)
UseTagsExpressionBacterialMutationPromoterN/AAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKRaION1-N1
Plasmid#177801PurposeMammalian expression of green fluorescent potassium indicator KRaION1DepositorInsertKRaION1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEIAV-SIN6.1 CBLucWm1A
Plasmid#44163DepositorInsertfirefly luciferase
UseLentiviral and LuciferaseTagsExpressionMutationPromoterCB (CMV enhancer/chicken beta-actin promoter)Available sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
SPLICS LY-MT Long P2A
Plasmid#213613PurposeDetect the long-range Lysosome-Mitochondria contactDepositorInsertSPLICS LY-MT Long P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
IMS-APEX2
Plasmid#79058PurposeExpresses APEX in the mitochondrial intermembrane space.DepositorInsertAPEX
UseTagsV5ExpressionBacterial and MammalianMutationPromoterT7 promoterAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
RN3P-KDM5B-FLAG
Plasmid#86398PurposeFLAG-tagged mouse KDM5B cDNA clone for mammalian cell expressionDepositorInsertKDM5B (Kdm5b Mouse)
UseTags3x-FLAGExpressionMammalianMutationPromoterLacAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BiP-mGFP
Plasmid#62231PurposeEncodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells.DepositorInserthamster BiP-mGFP-KDEL
UseTagsmGFPExpressionMammalianMutationPromotercmvAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1658-dCas9-EGFP
Plasmid#51023PurposeTemplate for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)DepositorInsertdCas9 fuse to EGFP
UseCRISPR and RetroviralTagsEGFPExpressionMammalianMutationPromoterMSCV LTR promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiSSTe4_GCaMP6f
Plasmid#213947PurposeAAV construct expressing GCaMP6f driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_GCaMP6fDepositorInsertGCaMP6f
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LIC
Plasmid#26094PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHexahistidine tag with Thrombin cleavageExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-hRXRα
Plasmid#135910PurposeHuman Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 PromoterDepositorInsertHuman retinoid X receptor- alpha (RXRA Human)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ESR1
Plasmid#116737PurposeLentiviral expression of ESR1DepositorInsertESR1 (ESR1 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
AYBEv3
Plasmid#193967PurposeAn engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertecTadA8e-nSpCas9(D10A)-MPG(v3)
UseTagsExpressionMammalianMutationG163R, N169S, S198A, K202A, G203A, S206A, K210APromoterhU6, EF1a, CBhAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-3C-sGFP11_pcDNA3.1(-)
Plasmid#182932PurposeFor expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragmentDepositorTypeEmpty backboneUseTags3C-cleavable split GFP 11, FLAG tag, and HA signa…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDuet TDP43 WT
Plasmid#27462DepositorInsertTDP-43 (TARDBP Human)
UseCoexpressionTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-cPLA2-mEGFP
Plasmid#184718PurposeExogenous cPLA2WTDepositorInsertcPLA2-mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MF85_PB-14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
Plasmid#176629PurposeTF C self-activation cassette in MultiFate-3DepositorInsert14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro-hMGT
Plasmid#172393PurposeRetroviral expression vector for human direct cardiac reprogrammingDepositorUseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VSVG
Plasmid#64084PurposeExpression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein geneDepositorInsertVSV glycoprotein
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK- dCas9-SunTag-P2A-HygR
Plasmid#193137PurposeExpresses dCas9 fused to the SunTag scaffoldDepositorInsertdCas9-SunTag-T2A-HygR
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PalmGRET
Plasmid#158221PurposeBRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.DepositorInsertPalmGFP-Nluc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-nCas9n
Plasmid#46757DepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionBacterialMutationPromoterT3Available sinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-HF1
Plasmid#138556PurposeExpresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-HF1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926APromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cherry-Fibrillarin-C1
Plasmid#134539PurposeExpresses Cherry-Fibrillarin fusion proteinDepositorInsertFibrillarin (FBL Human)
UseTagsCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF2538
Plasmid#144036PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX4 (SOX4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
1136- Desmoplakin-GFP
Plasmid#32227DepositorInsertDesmoplakin (DSP Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRIP3-mCherry
Plasmid#61386PurposeExpresses RIP3-mCherry in mammalian cellsDepositorInsertRIP3 (RIPK3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
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VHL-pGex2TK
Plasmid#20790DepositorInsertVHL (VHL Human)
UseTagsGSTExpressionBacterialMutationVHL wt cDNA, no mutationsPromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pFB1_hMSH2
Plasmid#129423PurposeExpression human MSH2 protein using baculovirusDepositorInserthuman MSH2 (MSH2 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrin promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST_119_LVL2 cam
Plasmid#179333PurposeNT-CRISPR plasmid for integration of multiple gRNAs.DepositorInsertPtac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLuc
Plasmid#170575PurposeExpress firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay.DepositorInsertLuc
UseLuciferase and RetroviralTagsNAExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF2971
Plasmid#144447PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR1H4 (NR1H4 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA AKT1
Plasmid#78778PurposeTo overexpress AKT1 in Mammalian CellsDepositorInsertAKT1 (AKT1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
NLSx3-CRY2PHR-p65
Plasmid#64124PurposePhotoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.DepositorInsertCRY2PHR-p65
UseTagsHis, NLSx3, and V5ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLY017SB_pAAV-U6sg(BbsI)-EFS-Thy1.1-P2A-SB100X
Plasmid#192151PurposeT cell CRISPR AAV-SB vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6c:sgRNA#4
Plasmid#64248Purposeexpresses sgRNA under U6c promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
mu-FLT3Lv1-pTRIP
Plasmid#163519PurposeUsed to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cellsDepositorInsertmurine-FLT3L variant 1 (Flt3l Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2X
Plasmid#75286PurposeExpresses proteins in the cytoplasm as fusions to maltose-binding proteinDepositorTypeEmpty backboneUseTagsmaltose-binding proteinExpressionBacterialMutationPromotertacAvailable sinceJuly 18, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMKV057
Plasmid#133312PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, WUS2 and IPTDepositorInsertLuc+. WUS2, IPT
UseTagsExpressionPlantMutationPromoterAtUbi10, Nos, 35SAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-ASC1
Plasmid#75134PurposeExpresses N-terminal Flag-tagged ASC1 in mammalian cellsDepositorInsertPYD and CARD domain containing (Pycard Mouse)
UseTagsFLAGExpressionMammalianMutationF23S (please see depositor comments below)PromoterCMVAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAT003
Plasmid#171628Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-TRAC
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCAG and EF1-aAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC22A5
Plasmid#132048PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC22A5 (SLC22A5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRDA_426
Plasmid#179097PurposeBE Cas expressionDepositorInsertABE8e
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-As
Plasmid#86196PurposeAsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processingDepositorInsertAsCpf1 gRNA cloning site for ribozyme cleavage
UseCRISPRTagsHammerhead ribozyme and HDV ribozymeExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA neo
Plasmid#104992PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD4
Plasmid#20186DepositorInsertNotch 4 Intracellular Domain (Notch4 Mouse)
UseTags3X FlagExpressionMammalianMutationContains murine Notch 4 Intracellular Domain (Val…PromoterCMVAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1 151-241
Plasmid#24390DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationContains amino acids 151-241.PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1 243-450
Plasmid#24392DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationContains amino acids 243-450.PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only