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Plasmid#211463PurposeLentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)DepositorInsertApaLI-dead
UseLentiviralTagsFLAGExpressionMammalianMutationD211A, E225A, K227APromoterTREAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
NSP4-1x-BioID2 pBabe-puro (SARS-CoV-2)
Plasmid#185998PurposeExpresses SARS-CoV-2 NSP4 fused to BioID2 and HA tagDepositorInsertSARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2)
UseRetroviralTagsBioID2 and HAExpressionMammalianMutationPromoterLTRAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro GEM-LTB4
Plasmid#202641PurposeExpresses the LTB4 sensor GEM-LTB4 in mammalian cellsDepositorInsertGEM-LTB4
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) IGF1R
Plasmid#107499PurposeExpresses IGF1R, puromycin resistantDepositorInsertIGF-1R (IGF1R Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-CDK6-mutPAM-PGK-puro
Plasmid#187638Purposesg resistant CDK6 ORFDepositorInsertCDK6 (CDK6 Human)
UseLentiviralTagsExpressionMammalianMutationPAM site mutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-ActA-IRES-puromycin-pLVx-EF1a
Plasmid#177433PurposeTo express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZIP-hCMV-ZsGreen-P2A-Puro-NT4
Plasmid#123328PurposeLentiviral vector for expression of non-targeting control shRNA #4 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insertDepositorInsertnon-targeting control shRNA embedded in miR30 backbone
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) MXRA7
Plasmid#107500PurposeExpresses MXRA7, puromycin resistantDepositorInsertMXRA7 (codon optimized) (MXRA7 Human)
UseLentiviralTagsExpressionMammalianMutationCodon optimized sequencePromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(D256A)-IRES-puro
Plasmid#128677Purposemammalian expression of Flag-Polß(D256A) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationD256APromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-Myc-DDK-IRES-Puro HNRNPQ
Plasmid#200729PurposeLentiviral expression of Flag-Tagged human HNRNPQDepositorInsertHNRNPQ (SYNCRIP Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
N113 nLV EF-1a-Gal4-Stop-PGK-Puro
Plasmid#44176DepositorInsertGal4/DBD (1-147)
UseLentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR
Plasmid#207355PurposeLentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.DepositorInsertRNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode1
Plasmid#83678Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode1
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
tetO-GFPnls-mPGK-rtTA-IRES-Puro
Plasmid#69545PurposeTet-On system expressing GFP tagged with 2x nuclear localization signals.DepositorInserteGFP
UseLentiviralTags2x nuclear localization sequence and T2A "sk…ExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) eGFP
Plasmid#107505PurposeExpresses eGFP, puromycin resistantDepositorInserteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT D554N (Puro)
Plasmid#154292PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationD554N mutation (Ser/Thr glycosylation-incompetent)PromoterPGKAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP
Plasmid#75123PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tagDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTags3x FLAG-tagExpressionMutationbromodomain replaced with the first bromodomain o…PromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G
Plasmid#157986PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
NSP6-1x-BioID2 pBabe-puro (SARS-CoV-2)
Plasmid#186000PurposeExpresses SARS-CoV-2 NSP6 fused to BioID2 and HA tagDepositorInsertSARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2)
UseRetroviralTagsBioID2 and HAExpressionMammalianMutationPromoterLTRAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
ORF3a-1x-BioID2 pBabe-puro (SARS-CoV-2)
Plasmid#185989PurposeExpresses SARS-CoV-2 ORF3a fused to BioID2 and HA tagDepositorInsertSARS-CoV-2 ORF3a (ORF3a SARS-CoV-2)
UseRetroviralTagsBioID2 and HAExpressionMammalianMutationPromoterLTRAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only