Addgene

We narrowed to 3,281 results for: mak

Showing: 2801 - 2820 of 3281 results

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TALED_Left-stuffer-8e

Plasmid

#187162

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

stuffer-8e

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Left-stuffer-E1347A

Plasmid

#187161

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Left-stuffer-E1347A

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Left-stuffer-1397N-UGI

Plasmid

#187174

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Left-stuffer-1397N-UGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Right-stuffer-1397C-UGI

Plasmid

#187171

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-1397C-UGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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HOXC6 (human) HIS-tag pET

Plasmid

#8565

Depositor

Corey Largman

Insert

HOXC6 (HOXC6 Human)

Use

Tags

His and T7

Expression

Bacterial

Mutation

This protein starts at an internal Met initially …

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
NmCherry-SpVasa

Plasmid

#66786

Purpose

expression of mCherry in Sea Urchin germ cells

Depositor

Amro Hamdoun

Article

Campanale et al Development. 2012 Feb;139(4):783-92. doi

Insert

Vasa

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

SP6

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO(Neo)-Venus-Flag-WT wobbled cdc20 (1991)

Plasmid

#61848

Purpose

for making stable RPE cells expressing WT cdc20

Depositor

Jonathon Pines

Insert

cdc20 (CDC20 Human)

Use

Tags

Flag and Venus

Expression

Mammalian

Mutation

wobbled

Promoter

CMV

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
mCherry pBSDONR P4r-P2

Plasmid

#121528

Purpose

For making C-terminal mCherry fusions using multisite Gateway cloning

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pRevLCav2

Plasmid

#60806

Purpose

used to make mutations in yeast REV3

Depositor

Thomas Kunkel, Youri Pavlov

Article

Pavlov et al Genetics. 2001 Sep;159(1):47-64.

Insert

REV3 (REV3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

contains 647 C-terminal amino acids and 335 bp of…

Promoter

Available since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode83

Plasmid

#83760

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode83

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pNP154

Plasmid

#106360

Purpose

A vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr II.

Depositor

Jonathan Ewbank

Article

Taffoni et al Elife. 2020 Jan 29;9. pii: 45047. doi: 1

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) no-tags pET

Plasmid

#8493

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HoxA9 (Hoxa9 Mouse)

Use

Tags

Expression

Bacterial

Mutation

makes protein by Western

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pKJ458

Plasmid

#228709

Purpose

Mammalian expression of Antibody C143 HC R39K (heavy chain); to be used with pKJ460 (Plasmid 228710) to make the antibody

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Science 0, eadr6006

Insert

C143 HC R39K

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ460

Plasmid

#228710

Purpose

Mammalian expression of Antibody C143 LC N28R (light chain); to be used with pKJ458 (Plasmid 228709) to make the antibody

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Science 0, eadr6006

Insert

C143 LC N28R

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ423

Plasmid

#228708

Purpose

Mammalian expression of Anti-CD71 LC (light chain); to be used with pKJ455 (Plasmid 228707) to make the antibody

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Science 0, eadr6006

Insert

Anti-CD71 LC

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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mhc2dab:GFP-LT

Plasmid

#68996

Purpose

for making transgenic fish

Depositor

David Traver

Article

Wittamer et al Blood. 2011 Jun 30;117(26):7126-35. doi:

Insert

mhc2dab (mhc2dab Zebrafish)

Use

Zebrafish expression

Tags

GFP-LT

Expression

Mutation

Promoter

mhc2dab

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pATT-BsaI-Pmag-NmagHigh1

Plasmid

#202713

Purpose

Contains Magnet flanked by linkers and BsaI sites insert for transposon insertion libraries.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

linker-pMag-RBS-nMagHigh1-linker

Use

Staging plasmid to make transposon insertion libr…

Tags

Expression

Mutation

Promoter

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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mhc2dab:mCherry

Plasmid

#68997

Purpose

for making transgenic fish

Depositor

David Traver

Article

Wittamer et al Blood. 2011 Jun 30;117(26):7126-35. doi:

Insert

mhc2dab (mhc2dab Zebrafish)

Use

Zebrafish expression

Tags

mcherry

Expression

Mutation

Promoter

mhc2dab

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pL0R_lacZ

Plasmid

#202095

Purpose

Receiver cloning vector for making level 0 parts. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pGWT35S-TP-UnaG

Plasmid

#203484

Purpose

For particle bombardment. A plastid transit peptide fused UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused UnaG

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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