pRevLCav2
(Plasmid
#60806)
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Purposeused to make mutations in yeast REV3
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Depositing Labs
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60806 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepYIY118
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Backbone manufacturerthis lab
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Modifications to backboneURA3 gene cloned into BstZ17-I site of the low copy number plasmid pMBL18.
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameREV3
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SpeciesS. cerevisiae (budding yeast)
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Mutationcontains 647 C-terminal amino acids and 335 bp of downstream noncoding sequence
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Entrez GeneREV3 (a.k.a. YPL167C, PSO1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (destroyed during cloning)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer M13pUC-fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRevLCav2 was a gift from Thomas Kunkel & Youri Pavlov (Addgene plasmid # 60806 ; http://n2t.net/addgene:60806 ; RRID:Addgene_60806) -
For your References section:
In vivo consequences of putative active site mutations in yeast DNA polymerases alpha, epsilon, delta, and zeta. Pavlov YI, Shcherbakova PV, Kunkel TA. Genetics. 2001 Sep;159(1):47-64. PubMed 11560886
Map uploaded by the depositor.
