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Plasmid#166148PurposeGateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.DepositorArticleTypeEmpty backboneUseTagsHis10-Smt3 and SNAPExpressionBacterialMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only
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pDEST17-His10-Smt3-DDX5(kf)-SNAP
Plasmid#166149PurposeExpression of N. furzeri DDX5 in E. coliDepositorArticleInsertDDX5
UseTagsHis10-Smt3 and SNAPExpressionBacterialMutationPromoterT7Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-DDX5(kf)-SNAP
Plasmid#166152PurposeExpression of N. furzeri DDX5 in E. coliDepositorArticleInsertDDX5
UseTagsHis10 and SNAPExpressionBacterialMutationPromoterT7Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxEXPr6
Plasmid#218290PurposeIntroduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3DepositorInserthph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJQR3
Plasmid#218289PurposeIntroduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6DepositorInsertHO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036WUseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pITEdv1
Plasmid#218283PurposeUse together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locusDepositorInsertHph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pITEu2
Plasmid#218282PurposeUse together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locusDepositorInsertHO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJE13HD7
Plasmid#218281PurposeUse together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.DepositorInsertHO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
mouse b6 full length
Plasmid#217816PurposeFull length mouse integrin b6 expressionDepositorInsertITGB6 (Itgb6 Mouse)
UseTagsP2A-mCherryExpressionMammalianMutationPromoterCAGAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
mouse b3 full length
Plasmid#217814PurposeFull length mouse integrin b3 expressionDepositorInsertITGB3 (Itgb3 Mouse)
UseTagsP2A-mCherryExpressionMammalianMutationPromoterCAGAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
mouse a2b full length
Plasmid#217812PurposeFull length mouse integrin a2b expressionDepositorInsertITGA2B (Itga2b Mouse)
UseTagsP2A-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
mouse av full length
Plasmid#217809PurposeFull length mouse integrin av expressionDepositorInsertITGAV (Itgav Mouse)
UseTagsP2A-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
eGFP-SUN1ΔN
Plasmid#213508PurposeExpresses human eGFP-SUNΔN in mammalian cellsDepositorInserteGFP-SUNΔN
UseTagsExpressionMammalianMutationSUN1ΔN has the first 138 amino acid (lamina domai…PromoterCMVAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
KU70-eGFP-SUN1ΔN
Plasmid#213516PurposeExpresses human KU70-eGFP-SUN1ΔN in mammalian cellsDepositorInsertKU70-eGFP-SUNΔN
UseTagsExpressionMammalianMutationSUN1ΔN has the first 138 amino acid (lamina domai…PromoterCMVAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT314 AAV-Rapamycin Inducible-NZ-Citrine
Plasmid#202063PurposeInducible AAV activatorDepositorArticleInsertAAV-Rapamycin Inducible-NZ-Citrine
UseAAV; Aav with reporterTagsExpressionMammalianMutationITR deletionPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT315 AAV-Rapamycin Inducible-ZNF473KRAB-Citrine
Plasmid#202064PurposeInducible AAV activatorDepositorArticleInsertAAV-Rapamycin Inducible-ZNF473KRAB-Citrine
UseAAV; Aav with reporterTagsExpressionMammalianMutationITR deletionPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT410 AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA
Plasmid#202059PurposeInducible AAV activatorDepositorArticleInsertAAV-Rapamycin Inducible-NFZ-Citrine with bGH pA
UseAAV; Aav with reporterTagsExpressionMammalianMutationITRs intactPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT313 AAV-Rapamycin Inducible-ZF-Citrine
Plasmid#202062PurposeInducible AAV activatorDepositorArticleInsertAAV-Rapamycin Inducible-ZF-Citrine
UseAAV; Aav with reporterTagsExpressionMammalianMutationITR deletionPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL
Plasmid#217676PurposeAAV-mediated, Cre-dependent co-expression of GtACR2 and soma-targeted Voltron2 (ORCHID) for assessing inhibitory receptor driving force through voltage imaging with concurrent activation of GtACR2.DepositorInsertGtACR2-P2A-Voltron2ST
UseAAVTagsSoma targeting sequence (Kv2.1) on Voltron2 gene …ExpressionMammalianMutationPromoterEF‐1αAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
mouse b8 full length
Plasmid#217817PurposeFull length mouse integrin b8 expressionDepositorInsertITGB8 (Itgb8 Mouse)
UseTagsP2A-mCherryExpressionMammalianMutationPromoterCAGAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only