KU70-eGFP-SUN1ΔN
(Plasmid
#213516)
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PurposeExpresses human KU70-eGFP-SUN1ΔN in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213516 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerThermo Fisher
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 8842
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKU70-eGFP-SUNΔN
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SpeciesH. sapiens (human)
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Insert Size (bp)4905
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MutationSUN1ΔN has the first 138 amino acid (lamina domain) of the WT removed. It contains a silent mutation of SUN1ΔN's 861th nucleotide (C->T). It confers resistance to a siSUN1 with a target sequence of CCGTGTTGAATTGGGCAAGCA.
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GenBank IDNM_001288976.2 AB648918.1
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CGC AAA TGG GCG GTA GGC GTG
- 3′ sequencing primer TAG AAG GCA CAG TCG AGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please see https://www.biorxiv.org/content/10.1101/2023.05.07.539750v1 for bioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KU70-eGFP-SUN1ΔN was a gift from Karim Mekhail (Addgene plasmid # 213516 ; http://n2t.net/addgene:213516 ; RRID:Addgene_213516) -
For your References section:
DNA double-strand break-capturing nuclear envelope tubules drive DNA repair. Shokrollahi M, Stanic M, Hundal A, Chan JNY, Urman D, Hakem A, Garcia RE, Hao J, Maass PG, Dickson BC, Hande MP, Pujana MA, Hakem R, Mekhail K. bioRxiv 2023.05.07.539750 10.1101/2023.05.07.539750