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AKT-translocation-mStrawberry reporter

Plasmid

#158685

Purpose

This plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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GIV-WT - FLAG

Plasmid

#65947

Purpose

Expression GIV/Girdin in Mammalian cell

Depositor

Pradipta Ghosh

Article

Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

Insert

GIV/Girdin (CCDC88A Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
human p53-(94-312)

Plasmid

#24866

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 94-312.

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
pgRNA-modified

Plasmid

#84477

Purpose

guide RNA expression lentiviral construct

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

guide RNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pET283xFlagPP7CPHis

Plasmid

#28174

Depositor

Kathleen Collins

Insert

PP7 coat protein optimized

Use

Tags

6xhistidine and triple FLAG peptide

Expression

Bacterial

Mutation

C68A,C73A

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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hCas9

Plasmid

#41815

Purpose

Expresses human codon optimized Cas9 nuclease for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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HA-VHL-pRc/CMV

Plasmid

#19999

Depositor

William Kaelin

Article

Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

wild type

Promoter

Available since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-DogTag

Plasmid

#105629

Purpose

Plasmid for recombinant expression in bacteria of SUMO domain fused to DogTag, permitting covalent linkage to SnoopTagJr

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

SUMO-DogTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3

Plasmid

#21073

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

First ATG is not included.

Promoter

CMV

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pAd-Wnt3a

Plasmid

#12518

Depositor

Tong-Chuan He

Article

Luo et al J Biol Chem. 2004 Dec 31. 279(53):55958-

Insert

Wnt3A (Wnt3a Mouse)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2006

Availability

Academic Institutions and Nonprofits only
CX-nestinGFPas

Plasmid

#32401

Depositor

Patrick Salmon, Henryk Zulewski

Article

Eberhardt et al Biochem Biophys Res Commun. 2006 Jul 7;3

Insert

nestin promoter/enhancer GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-pCMV-PfV-GS-Sapphire

Plasmid

#116933

Purpose

Mammalian vector for expression of the 40nm GEM

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Use

Tags

Sapphire

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-p53

Plasmid

#12091

Depositor

Tyler Jacks

Article

Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

Insert

p53 (TP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

72R variant of p53

Promoter

Available since

July 5, 2006

Availability

Academic Institutions and Nonprofits only

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pFastBac1_FLAG-BPTF

Plasmid

#102242

Purpose

Expression of human Flag-BPTF using baculovirus from pFastBac1

Depositor

Joe Landry

Insert

Flag-BPTF (BPTF Human)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

Polyhedron

Available since

Dec. 11, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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hMEF2A WT

Plasmid

#118354

Purpose

This plasmid expresses human MEF2A-1 WT.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hMEF2A (MEF2A Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

WT

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherryC2-Gephyrin P1

Plasmid

#68820

Purpose

mCherry tagged Geph for mammalian expression

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al Proc Natl Acad Sci U S A. 2011 Jan 4;108

Insert

Gephyrin (Gphn Rat)

Use

Tags

mCherry

Expression

Mammalian

Mutation

silent mutations on internal EcoRI site. Gephyrin…

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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mVenus N1

Plasmid

#27793

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Has Service

Cloning Grade DNA

Insert

mVenus N1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2011

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF2-N2L

Plasmid

#21163

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Nanog, Lin28 (NANOG, LIN28A Human)

Use

Lentiviral; Reprogramming

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pMXs-3XMyc-EGFP-OMP25

Plasmid

#83355

Purpose

For tagging mitochondria with Myc epitopes (Control-MITO construct)

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XMyc-EGFP-OMP25

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pSBtet-GP

Plasmid

#60495

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

TCE & RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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