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Plasmid#102459PurposeExpresses a fusion protein of thermostabilized β1 adrenergic receptor and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.DepositorArticleInsertβ1AR
UseTagsHis tag and sfGFPExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW217
Plasmid#113239PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->ggtaCPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW215
Plasmid#113237PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->ggAagPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW214
Plasmid#113236PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->gCtagPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW213
Plasmid#113235PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Cgtag mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->CgtagPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW211
Plasmid#113233PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->AACGADepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->AACGAPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW216
Plasmid#113238PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->ggtTgPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
KHT-1
Plasmid#113054PurposeExpresses worm KHT-1 in mammalian cellsDepositorInsertK+ channel for Habituation to Tap subunit 1
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP4GY-cccdB
Plasmid#83878PurposeDestination vector for EYFP fusions integrated at yeast Trp locus, expression from the GPD promoterDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-Mb-crRNA
Plasmid#78959PurposeCloning vector for expression of MbCpf1 crRNA. It contains BsmB1 site for cloning.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-CBPNDHFR
Plasmid#63995PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsCalmodulin Binding Peptide (CBP); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-HPCHISNDHFR
Plasmid#63984PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Protein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-HPCHISNDHFR
Plasmid#63948PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Protein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-CBPHISNDHFR
Plasmid#63945PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Calmodulin Binding Peptide (CBP); TEV…ExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-STRHISNDHFR
Plasmid#63982PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Strep II (STR); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-CBPNDHFR
Plasmid#63977PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsCalmodulin Binding Peptide (CBP); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-STRHISNDHFR
Plasmid#63964PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Strep II (STR); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-CBPHISNDHFR
Plasmid#63963PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Calmodulin Binding Peptide (CBP); TEV…ExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-C
Plasmid#60979PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic ceruleanDepositorInsertCerulean
UseZebrafishTagsExpressionMutationPromoterBactin2Available sinceFeb. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab2(1-214)
Plasmid#59550Purposemakes 6x Fusion of Full length ceRab2DepositorInsert1-214
UseTagsExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only